Recombinant Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6886(B)] to Methionine Aminopeptidase 2/p67
- Suitable for: Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)]
See all Methionine Aminopeptidase 2/p67 primary antibodies -
Description
Rabbit monoclonal [EPR6886(B)] to Methionine Aminopeptidase 2/p67 -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt (Intra), WBmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human Methionine Aminopeptidase 2/p67. The exact sequence is proprietary.
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Positive control
- HepG2, 293T, HeLa, C6, RAW 264.7, PC12 and NIH 3T3 cell lysates; Permeabilized 293T cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6886(B) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab124953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa).
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Notes |
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Flow Cyt (Intra)
1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
1/1000 - 1/10000. Detects a band of approximately 67 kDa (predicted molecular weight: 52 kDa). |
Target
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Function
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). The catalytic activity of human METAP2 toward Met-Val peptides is consistently two orders of magnitude higher than that of METAP1, suggesting that it is responsible for processing proteins containing N-terminal Met-Val and Met-Thr sequences in vivo.
Protects eukaryotic initiation factor EIF2S1 from translation-inhibiting phosphorylation by inhibitory kinases such as EIF2AK2/PKR and EIF2AK1/HCR. Plays a critical role in the regulation of protein synthesis. -
Sequence similarities
Belongs to the peptidase M24A family. Methionine aminopeptidase eukaryotic type 2 subfamily. -
Post-translational
modificationsContains approximately 12 O-linked N-acetylglucosamine (GlcNAc) residues. O-glycosylation is required for EIF2S1 binding. -
Cellular localization
Cytoplasm. About 30% of expressed METAP2 associates with polysomes. - Information by UniProt
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Database links
- Entrez Gene: 10988 Human
- Entrez Gene: 56307 Mouse
- Entrez Gene: 64370 Rat
- Omim: 601870 Human
- SwissProt: P50579 Human
- SwissProt: O08663 Mouse
- SwissProt: P38062 Rat
- Unigene: 444986 Human
see all -
Alternative names
- A930035J23Rik antibody
- AI047573 antibody
- AL024412 antibody
see all
Images
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All lanes : Anti-Methionine Aminopeptidase 2/p67 antibody [EPR6886(B)] (ab124953) at 1/1000 dilution
Lane 1 : HepG2 cell lysates
Lane 2 : 293T cell lysates
Lane 3 : HeLa cell lysates
Lane 4 : C6 cell lysates
Lane 5 : RAW 264.7 cell lysates
Lane 6 : PC12 cell lysates
Lane 7 : NIH 3T3 cell lysates
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size: 52 kDa
Observed band size: 67 kDa why is the actual band size different from the predicted? -
ab124953 at 1/10 dilution staining Methionine Aminopeptidase 2/p67 in permeabilized 293T cells by intracellular flow cytometry (red) or a Rabbit IgG (negative control) (green).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab124953 has been referenced in 1 publication.
- Ng JY et al. Tyrosine nitration moderates the peptidase activity of human methionyl aminopeptidase 2. Biochem Biophys Res Commun 440:37-42 (2013). PubMed: 24041691