Anti-Methionine Sulfoxide Reductase A antibody (ab16803)

Overview

  • Product nameAnti-Methionine Sulfoxide Reductase A antibody
    See all Methionine Sulfoxide Reductase A primary antibodies
  • Description
    Rabbit polyclonal to Methionine Sulfoxide Reductase A
  • Tested applicationsSuitable for: ELISA, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
    Predicted to work with: Macaque Monkey
  • Immunogen

    Recombinant full length protein (Human).

  • Positive control
    • Mouse kidney extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab16803 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/2000. Predicted molecular weight: 26 kDa.
IHC-P Use a concentration of 1 µg/ml.

Target

  • FunctionHas an important function as a repair enzyme for proteins that have been inactivated by oxidation. Catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine.
  • Tissue specificityUbiquitous. Highest expression in adult kidney and cerebellum, followed by liver, heart ventricles, bone marrow and hippocampus.
  • Sequence similaritiesBelongs to the MsrA Met sulfoxide reductase family.
  • Cellular localizationCytoplasm; Cytoplasm. Nucleus and Mitochondrion.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cytosolic methionine S sulfoxide reductase antibody
    • Methionine sulphoxide reductase A antibody
    • Mitochondrial peptide methionine sulfoxide reductase antibody
    • MSR A antibody
    • msrA antibody
    • MSRA_HUMAN antibody
    • peptide met (O) reductase antibody
    • Peptide Met(O) reductase antibody
    • Peptide methionine (S) S oxide reductase antibody
    • Peptide-methionine (S)-S-oxide reductase antibody
    • PMSR antibody
    • Protein methionine S oxide reductase antibody
    • Protein-methionine-S-oxide reductase antibody
    see all

Anti-Methionine Sulfoxide Reductase A antibody images

  • All lanes : Anti-Methionine Sulfoxide Reductase A antibody (ab16803)

    Lane 1 : Mouse Kidney
    Lane 2 : Rat Kidney


    Predicted band size : 26 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)
  • ab16803 (1µg/ml) staining Methionine Sulfoxide Reductase A in human colon using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of nuclear/cytoplasmic compartment of the intestinal glands cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • Anti-Methionine Sulfoxide Reductase A antibody (ab16803) at 1/2000 dilution + Methionine Sulfoxide Reductase A protein (Active) (ab82728) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute

References for Anti-Methionine Sulfoxide Reductase A antibody (ab16803)

This product has been referenced in:
  • Zhang C  et al. Anoxia, acidosis, and intergenic interactions selectively regulate methionine sulfoxide reductase transcriptions in mouse embryonic stem cells. J Cell Biochem 112:98-106 (2011). WB ; Mouse . Read more (PubMed: 20872796) »
  • Brennan LA  et al. TXNL6 is a novel oxidative stress-induced reducing system for methionine sulfoxide reductase a repair of a-crystallin and cytochrome C in the eye lens. PLoS One 5:e15421 (2010). WB ; Human . Read more (PubMed: 21079812) »

See all 3 Publications for this product

Product Wall

Thank you for contacting us.

The product code of the antibody that was used in the reference listed on the ab16803 datasheet was, as far as we know, ab16803. I believe this information,which is not in the text of the paper, was given to us b...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Cow Recombinant protein (None)
Loading amount 0.15 µg
Specification None
Gel Running Conditions Reduced Denaturing (4-20% Gradient Pre-cast Gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 29 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"