Overview

  • Product nameAnti-Methionine Sulfoxide Reductase B antibody
    See all Methionine Sulfoxide Reductase B primary antibodies
  • Description
    Mouse monoclonal to Methionine Sulfoxide Reductase B
  • Tested applicationsSuitable for: ELISA, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Orangutan
  • Immunogen

    Recombinant fragment with tag: MSFCSFFGGE VFQNHFEPGV YVCAKCGYEL FSSRSKYAHS SPWPAFTETI HADSVAKRPE HNRSEALKVS CGKCGNGLGH EFLN, corresponding to N terminal amino acids 1-85 of Human Methionine Sulfoxide Reductase B

  • Positive control
    • Methionine Sulfoxide Reductase B transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66061 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 10 kDa (predicted molecular weight: 13 kDa).

Target

Anti-Methionine Sulfoxide Reductase B antibody images

  • All lanes : Anti-Methionine Sulfoxide Reductase B antibody (ab66061) at 1 µg/ml

    Lane 1 : Methionine Sulfoxide Reductase B transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 13 kDa
    Observed band size : 10 kDa (why is the actual band size different from the predicted?)

References for Anti-Methionine Sulfoxide Reductase B antibody (ab66061)

This product has been referenced in:
  • Schoenmakers E  et al. Mutations in the selenocysteine insertion sequence-binding protein 2 gene lead to a multisystem selenoprotein deficiency disorder in humans. J Clin Invest 120:4220-35 (2010). WB ; Human . Read more (PubMed: 21084748) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"