For the best experience on the Abcam website please upgrade to a modern browser such as
Rabbit polyclonal to Methionyl tRNA synthetase
Predicted to work with:
Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset
Synthetic peptide, corresponding to a region within amino acids 650-700 of Human Methionyl tRNA synthetase (NP_004981.2).
293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH3T3 cells whole cell lysates.
Shipped at 4°C. Store at +4°C.
Preservative: 0.09% Sodium azide Constituent: 99% Tris citrate/phosphate Note: pH 7-8.
Concentration information loading...
Immunogen affinity purified
ab157119 was affinity purified using an epitope specific to Methionyl tRNA synthetase immobilized on solid support.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/5000. Predicted molecular weight: 101 kDa.
Use at 2-10 µg/mg of lysate.
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain. Contains 1 WHEP-TRS domain.
Information by UniProt
Western blot - Anti-Methionyl tRNA synthetase antibody (ab157119)
All lanes : Anti-Methionyl tRNA synthetase antibody (ab157119) at 0.4 µg/ml Lane 1 : 293T whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : Jurkat whole cell lysate Lane 4 : Mouse TCMK1 whole cell lysate Lane 5 : Mouse NIH3T3 whole cell lysate Lysates/proteins at 50 µg per lane. Developed using the ECL technique Predicted band size : 101 kDa Exposure time : 3 minutes
Immunoprecipitation - Anti-Methionyl tRNA synthetase antibody (ab157119)
Detection of Methionyl tRNA synthetase by Western Blot of Immunprecipitate.
ab157119 at 1µg/ml labeling Methionyl tRNA synthetase in 293T whole cell lysate immunoprecipitated using ab157119 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"