Anti-Methionyl tRNA synthetase antibody (ab157119)

Overview

  • Product name
    Anti-Methionyl tRNA synthetase antibody
    See all Methionyl tRNA synthetase primary antibodies
  • Description
    Rabbit polyclonal to Methionyl tRNA synthetase
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IPmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Common marmoset
  • Immunogen

    Synthetic peptide, corresponding to a region within amino acids 650-700 of Human Methionyl tRNA synthetase (NP_004981.2).

  • Positive control
    • 293T, HeLa, Jurkat, mouse TCMK 1 and mouse NIH3T3 cells whole cell lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab157119 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 101 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

Images

  • All lanes : Anti-Methionyl tRNA synthetase antibody (ab157119) at 0.4 µg/ml

    Lane 1 : 293T whole cell lysate
    Lane 2 : HeLa whole cell lysate
    Lane 3 : Jurkat whole cell lysate
    Lane 4 : Mouse TCMK1 whole cell lysate
    Lane 5 : Mouse NIH3T3 whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 101 kDa


    Exposure time: 3 minutes
  • Detection of Methionyl tRNA synthetase by Western Blot of Immunprecipitate.
    ab157119 at 1µg/ml labeling Methionyl tRNA synthetase in 293T whole cell lysate immunoprecipitated using ab157119 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Detection: Chemiluminescence with exposure time of 30 seconds.

References

ab157119 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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