Anti-MetRS/MARS antibody [MARSD10B4] (ab50793)
Key features and details
- Mouse monoclonal [MARSD10B4] to MetRS/MARS
- Suitable for: WB, Flow Cyt, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-MetRS/MARS antibody [MARSD10B4]
See all MetRS/MARS primary antibodies -
Description
Mouse monoclonal [MARSD10B4] to MetRS/MARS -
Host species
Mouse -
Tested applications
Suitable for: WB, Flow Cyt, ICC/IF, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human MetRS/MARS aa 50-200 (C terminal).
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Positive control
- WB: NIH3T3 whole cell, HeLa whole cell and F2408 whole cell lysates. IP: RIPA lysate of HeLa cells. ICC: HeLa cell. Flow Cyt: Hela cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.03% Potassium phosphate, 0.1312% Sodium phosphate, 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Ab50793 was filtered through an 0.22 µm membrane. -
Clonality
Monoclonal -
Clone number
MARSD10B4 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
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Flow Cyt |
1/10.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/10.
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IP |
Use at 5 µg/mg of lysate.
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Notes |
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WB
1/100. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa). |
Flow Cyt
1/10. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/10. |
IP
Use at 5 µg/mg of lysate. |
Target
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Sequence similarities
Belongs to the class-I aminoacyl-tRNA synthetase family.
Contains 1 GST C-terminal domain.
Contains 1 WHEP-TRS domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 4141 Human
- Entrez Gene: 216443 Mouse
- Entrez Gene: 299851 Rat
- Omim: 156560 Human
- SwissProt: P56192 Human
- SwissProt: Q68FL6 Mouse
- Unigene: 632707 Human
- Unigene: 28173 Mouse
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Alternative names
- cytoplasmic antibody
- FLJ35667 antibody
- Mars antibody
see all
Images
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Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + HeLa whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 101 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted? -
Immunoprecipitation from 200ug RIPA lysate of HeLa cells using 5ug of ab50793. Visualized by Western Blot using ab50793 at 1/100 dilution, anti-mouse IgG secondary 1/1000 and ECL detection.
Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 5 µg + RIPA lysate of HeLa cells at 200 µg
Observed band size: 110 kDa why is the actual band size different from the predicted? -
Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody at 1/2500 dilution
Predicted band size: 101 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted? -
Anti-MetRS/MARS antibody [MARSD10B4] (ab50793) at 1/100 dilution + F2408 whole cell lysate at 50 µg
Secondary
Mouse IgG antibody
Predicted band size: 101 kDa
Observed band size: 98 kDa why is the actual band size different from the predicted? -
Ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cell by Immunocytochemistry/Immunofluorescence. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.
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Ab50793, at a dilution of 1/10, staining MetRS/MARS in Hela cells by Flow Cytometry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/400.
Protocols
Datasheets and documents
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Datasheet download
References (7)
ab50793 has been referenced in 7 publications.
- Theodoridis PR et al. Local translation in nuclear condensate amyloid bodies. Proc Natl Acad Sci U S A 118:N/A (2021). PubMed: 33568529
- Silva RC et al. A Rapid Extraction Method for mammalian cell cultures, suitable for quantitative immunoblotting analysis of proteins, including phosphorylated GCN2 and eIF2a. MethodsX 5:75-82 (2018). PubMed: 30619721
- Wang D et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep 25:398-412.e6 (2018). PubMed: 30304680
- Kwon NH et al. Stabilization of Cyclin-Dependent Kinase 4 by Methionyl-tRNA Synthetase in p16INK4a-Negative Cancer. ACS Pharmacol Transl Sci 1:21-31 (2018). PubMed: 32219202
- Park SJ et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . PubMed: 26544075
- Gulati P et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB, IP ; Mouse . PubMed: 23359686
- Yannay-Cohen N et al. LysRS serves as a key signaling molecule in the immune response by regulating gene expression. Mol Cell 34:603-11 (2009). WB ; Rat . PubMed: 19524539