Anti-Methionyl tRNA synthetase antibody [MARSD10B4] (ab50793)

Overview

  • Product name
    Anti-Methionyl tRNA synthetase antibody [MARSD10B4]
    See all Methionyl tRNA synthetase primary antibodies
  • Description
    Mouse monoclonal [MARSD10B4] to Methionyl tRNA synthetase
  • Tested applications
    Suitable for: WB, Flow Cyt, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant Human MARS

  • Positive control
    • WB: NIH3T3 whole cell, HeLa whole cell and F2408 whole cell lysates. IP: RIPA lysate of HeLa cells. ICC: HeLa cell. FC: Hela cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab50793 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/100. Detects a band of approximately 101 kDa (predicted molecular weight: 101 kDa).
Flow Cyt 1/10.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ICC/IF 1/10.
IP Use at 5 µg/mg of lysate.

Target

Images

  • Anti-Methionyl tRNA synthetase antibody [MARSD10B4] (ab50793) at 1/100 dilution + HeLa whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 101 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)
  • Immunoprecipitation from 200ug RIPA lysate of HeLa cells using 5ug of ab50793. Visualized by Western Blot using ab50793 at 1/100 dilution, anti-mouse IgG secondary 1/1000 and ECL detection.
  • Anti-Methionyl tRNA synthetase antibody [MARSD10B4] (ab50793) at 1/100 dilution + NIH3T3 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody at 1/2500 dilution

    Predicted band size : 101 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)
  • Anti-Methionyl tRNA synthetase antibody [MARSD10B4] (ab50793) at 1/100 dilution + F2408 whole cell lysate at 50 µg

    Secondary
    Mouse IgG antibody

    Predicted band size : 101 kDa
    Observed band size : 98 kDa (why is the actual band size different from the predicted?)
  • Ab50793, at a dilution of 1/10, staining Methionyl tRNA synthetase in Hela cell by Immunocytochemistry/Immunofluorescence. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/200.
  • Ab50793, at a dilution of 1/10, staining Methionyl tRNA synthetase in Hela cells by Flow Cytometry. AlexaFluor®488 Goat anti-mouse IgG was used as the secondary antibody at a dilution of 1/400.

References

This product has been referenced in:
  • Park SJ  et al. Evaluation of Multi-tRNA Synthetase Complex by Multiple Reaction Monitoring Mass Spectrometry Coupled with Size Exclusion Chromatography. PLoS One 10:e0142253 (2015). WB ; Human . Read more (PubMed: 26544075) »
  • Gulati P  et al. Role for the obesity-related FTO gene in the cellular sensing of amino acids. Proc Natl Acad Sci U S A 110:2557-62 (2013). WB, IP ; Mouse . Read more (PubMed: 23359686) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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