Anti-Methotrexate antibody (ab2328)

Overview

  • Product nameAnti-Methotrexate antibody
  • Description
    Goat polyclonal to Methotrexate
  • SpecificityThe antibody is highly specific for Methotrexate, but cross-reactivities have not been tested.
  • Tested applicationsSuitable for: ELISA, RIAmore details
  • Immunogen

    Pure Methotrexate conjugated to hemocyanin

Properties

Applications

Our Abpromise guarantee covers the use of ab2328 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
RIA
  • Application notesWorking dilutions are best determined by the end user.
  • Target

    • RelevanceMethotrexate (rINN) abbreviated MTX and formerly known as amethopterin, is an antimetabolite and antifolate drug used in treatment of cancer, autoimmune diseases and as an abortifacient in the induction of medical abortions. It acts by inhibiting the metabolism of folic acid. Methotrexate competitively inhibits dihydrofolate reductase (DHFR), an enzyme that participates in the tetrahydrofolate synthesis. The affinity of methotrexate for DHFR is about one thousand-fold that of folate for DHFR. Methotrexate acts specifically during DNA and RNA synthesis, and thus it is cytotoxic during the S-phase of the cell cycle. Logically, it therefore has a greater toxic effect on rapidly dividing cells which replicate their DNA more frequently, and thus inhibits the growth and proliferation of these non-cancerous cells. Lower doses of methotrexate have been shown to be very effective for the management of rheumatoid arthritis, Crohn's disease, and psoriasis.
    • Alternative names
      • Amethopterin antibody
      • MTX antibody

    References for Anti-Methotrexate antibody (ab2328)

    ab2328 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your email and kind answer. Indeed, I have found the below publication where the IgG content of normal adult goat serum was about 20mg/ml. http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1445503/ Also, yes, only the specific antibodie...

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    Thank you for your enquiry. I apologise for the delay in responding to your message. Since it is the antibody directed against methotrexate that you need to purify, you will not need to include the hemocyanin. IgG from the sample can be purified no...

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    Thank you for your enquiry. This antibody is not part of a kit, but we do have suggestions for compatible secondary antibodies and other products that may be helpful. These are available on the "Related Products" tab on the datasheet. I hope thi...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"