DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from cells by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.
|Components||24 tests||48 tests|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Anti-5-Methylcytosine (1 mg/mL)||1 x 25µl||1 x 50µl|
|Antibody Buffer||1 x 15ml||1 x 30ml|
|Binding Buffer||1 x 5ml||1 x 8ml|
|ChIP Dilution Buffer||1 x 4ml||1 x 6ml|
|DNA Release Buffer||1 x 2ml||2 x 2ml|
|Elution Buffer||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Lysis Buffer||1 x 4ml||1 x 6ml|
|Normal Mouse IgG (1 mg/mL)||1 x 10µl||1 x 20µl|
|Pre-Lysis Buffer||1 x 4ml||1 x 8ml|
|Proteinase K (10 mg/mL)||1 x 25µl||1 x 50µl|
|Reverse Buffer||1 x 2ml||2 x 2ml|
|Wash Buffer||1 x 28ml||2 x 28ml|
Our Abpromise guarantee covers the use of ab117135 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab117135 has not yet been referenced specifically in any publications.