DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.
Methylated DNA Immunoprecipitation (MeDIP) Kit - DNA (ab117133) enables the user to enrich methylated DNA by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.
NOTE: the size of this kit is based on number of TESTS not SAMPLES. A test simply refers to a single assay well. If you are not sure which size is most suitable for you, contact our Scientific Support Team.
|Components||24 tests||48 tests|
|8-Well Assay Strips (with Frame)||3 units||6 units|
|8-Well Strip Caps||3 units||6 units|
|Anti-5-Methylcytosine (1 mg/mL)||1 x 25µl||1 x 50µl|
|Antibody Buffer||1 x 8ml||1 x 16ml|
|Binding Buffer||1 x 5ml||1 x 8ml|
|DNA Release Buffer||1 x 2ml||1 x 4ml|
|Elution Buffer||1 x 0.6ml||1 x 1.2ml|
|F-Collection Tube||30 units||50 units|
|F-Spin Column||30 units||50 units|
|Normal Mouse IgG (1 mg/mL)||1 x 10µl||1 x 20µl|
|Proteinase K (10 mg/mL)||1 x 25µl||1 x 50µl|
|Reaction Buffer||1 x 4ml||1 x 8ml|
|Wash Buffer||1 x 16ml||2 x 16ml|
Our Abpromise guarantee covers the use of ab117133 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
MeDIP was performed using 1μg of DNA with ab117133. Real-time PCR was carried out with MeDIP DNAs and the frequency of DNA methylation of immunoprecipitated DNA vs input DNA was calculated.