Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue (ab117136)


  • Product nameMethylated DNA Immunoprecipitation (MeDIP) Kit - Tissue
    See all hydroxymethylated DNA kits
  • Sample type
  • Assay time
    3h 30m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position of a CpG dinucleotide which leads to epigenetic inactivation of genes when found in 5'-CpG-3'dinucleotides within promoters or in the first exon of genes. It has been demonstrated that DNA methylation plays an important role in the regulation of gene expression, tumorigenesis, and other genetic and epigenetic diseases, and that alterations in DNA methylation patterns are associated with cancer.

     Methylated DNA Immunoprecipitation (MeDIP) ChIP Kit (ab117135) enables the user to enrich methylated DNA directly from various mammalian tissues by using an antibody specific to methyl cytosine (5-mC) to immunoprecipitate methylated genomic DNA (MeDIP). The enriched methylated fractions can then be used in various downstream applications including qualitative and quantitative PCR, as well as southern blot and DNA microarray.

  • Tested applicationsSuitable for: Functional Studiesmore details



    Our Abpromise guarantee covers the use of ab117136 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.


    References for Methylated DNA Immunoprecipitation (MeDIP) Kit - Tissue (ab117136)

    ab117136 has not yet been referenced specifically in any publications.

    Product Wall

    I apologize again for the incorrect information given originally.

    For tissues we recommend, ab117136.
    In comparison, kit ab117135 would be used when starting with cells. When using a DNA sample, please use ab117133 instead.

    I ...

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    The ChiPed DNA amount with this kit is about 30-50 ng/well. Genome-wide amplification may be needed for some downstream applications such as microarray or sequencing. I hope this is helpful. Please let me know if you need anything further.

    I am sorry for the delay in our reply. Here are answers to your questions: 1. The antibodies included in ab117136, 117144, 117145, 117147, 117150 and117151 are not sold separately in our catalog. 2. The antibodies in kits ab117144, 117145 and117147 hav...

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    1. Yes, the ChIPed DNA is suitable for ChIP-Seq. Some customers report having improved ChIP-Seq results when the ChIPed DNA is further purified via phenol/chloroform extraction and ethanol precipitation. 2. The 'required volume' is unfortunately vague ...

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    This kit can be used for ChIP with adipose tissues. Here are the references for using our general tissue ChIP kit in adipose tissues: - Yue, al.(April 2009). Peroxisome proliferator-activated receptor {gamma} stimulation of adipocyte ApoE gene tr...

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