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Methylated DNA Quantification Kit (Colorimetric) (ab117128)

Overview

  • Product nameMethylated DNA Quantification Kit (Colorimetric)
    See all 5-methylcytosine kits
  • Detection methodColorimetric
  • Sample type
    Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Assay typeQuantitative
  • Sensitivity
    >= 0.2 ng/well
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Species independent
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

    Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

    It can be paired to use with Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab117128 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Methylated DNA Quantification Kit (Colorimetric) images

    • Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.

    Protocols

    References for Methylated DNA Quantification Kit (Colorimetric) (ab117128)

    This product has been referenced in:
    • Farias N  et al. The effects of folic acid on global DNA methylation and colonosphere formation in colon cancer cell lines. J Nutr Biochem 26:818-26 (2015). Functional Studies ; Human . Read more (PubMed: 25804133) »
    • Panikar CS  et al. Presence of DNA methyltransferase activity and CpC methylation in Drosophila melanogaster. Mol Biol Rep N/A:N/A (2015). Functional Studies ; Fruit fly (Drosophila melanogaster) . Read more (PubMed: 26547851) »

    See all 2 Publications for this product

    Product Wall


    I am very pleased to hear you would like to accept our offer and test ab117128 with "Flatworm species". This code will give you €350 off your next order before the expiration date. To redeem this offer, please submit an Abreview for...

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    Tween at 0.05 - 0.1% should be used.

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    I heard back from the lab that generally, PBS-T buffer may be used for washing, however for the best results, they recommend to use the wash buffer included in the kit. We could get the washing buffer (either 14 mL...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"