Methylated DNA Quantification Kit (Colorimetric) (ab117128)

Overview

  • Product nameMethylated DNA Quantification Kit (Colorimetric)See all 5-methylcytosine kits ...
  • Sample type
    Saliva, Urine, Serum, Plasma, Tissue, Adherent cells, Suspension cells
  • Sensitivity
    >= 0.2 ng/well
  • Assay time
    4h 00m
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: all Mammals
  • Product overview

    DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.

     Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.

    It can be paired to use with EpiSeeker hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneously quantification of both methylated and hydroxymethylated DNA.

  • Tested applicationsFunctional Studiesmore details

Properties

    Applications

    Our Abpromise guarantee covers the use of ab117128 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    Functional Studies Use at an assay dependent concentration.

    Methylated DNA Quantification Kit (Colorimetric) images

    • Demonstration of high sensitivity and specificity of methylated DNA detection achieved using ab117128. Synthetic unmethylated DNA (contains 50% of cytosine) and methylated DNA (contains 50% of 5-methylcytosine) were added into the assay wells at different concentrations and then measured with ab117128.

    Protocols

    References for Methylated DNA Quantification Kit (Colorimetric) (ab117128)

    ab117128 has not yet been referenced specifically in any publications.

    Product Wall


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    Tween at 0.05 - 0.1% should be used.

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    I heard back from the lab that generally, PBS-T buffer may be used for washing, however for the best results, they recommend to use the wash buffer included in the kit. We could get the washing buffer (either 14 mL...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"