DNA methylation is a covalent modification of the cytosine ring at the 5' position, resulting in 5-methylcytosine (5-mC). In somatic cells, 5-mC is found almost exclusively in the context of paired symmetrical methylation of the dinucleotide CpG. The biological important of 5-mC as a major epigenetic modification in phenotype and gene expression has been recognized widely. Quantification of 5-mC content or global methylation status in cells such as tumor cells could provide very useful information for detection and analysis of cancer.
Methylated DNA Quantification Kit (Colorimetric) (ab117128) is a complete set of optimized buffers and reagents to quantify global DNA methylation by specifically measuring levels of 5-methylcytosine (5-mC) in a microplate-based format.
It can be paired to use with Abcam's Hydroxymethylated DNA Quantification Kit (Colorimetric) (ab117130), which specifically quantifies 5-hmC, for simultaneous quantification of both methylated and hydroxymethylated DNA.
|Components||48 tests||96 tests|
|10X Wash Buffer||1 x 14ml||1 x 28ml|
|8-Well Assay Strips (with Frame)||6 units||12 units|
|Binding Solution||1 x 5ml||1 x 10ml|
|Capture Antibody, 1000 µg/ml||1 x 4µl||1 x 8µl|
|Detection Antibody, 400 µg/ml||1 x 8µl||1 x 16µl|
|Developer Solution||1 x 5ml||1 x 10ml|
|Enhancer Solution||1 x 8µl||1 x 16µl|
|Negative Control, 20 µg/ml||1 x 10µl||1 x 20µl|
|Positive Control, 20 µg/ml||1 x 10µl||1 x 20µl|
|Stop Solution||1 x 5ml||1 x 10ml|
Our Abpromise guarantee covers the use of ab117128 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|