The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at 5 µg/mg of lysate.
RelevanceMany proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants.
Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
ab76118 staining Methylated Lysine in Human Breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Western blot - Methylated Lysine antibody (ab76118)
All lanes : Anti-Methylated Lysine antibody (ab76118)
Lane 1 : Total protein from Human melanoma cell line (MMRU) Lane 2 : Total protein from Human melanoma cell line (MMRU) Lane 3 : Total protein from Human melanoma cell line (MMRU) with N-(epsilon) trimethyllysine at 50 µg/ml Lane 4 : Total protein from Human melanoma cell line (MMRU) with N-(epsilon) trimethyllysine at 50 µg/ml
References for Anti-Methylated Lysine antibody (ab76118)
This product has been referenced in:
Alban C et al. Uncovering the protein lysine and arginine methylation network in Arabidopsis chloroplasts. PLoS One9:e95512 (2014).
Arabidopsis thaliana, Spinacia oleracea, Pisum sativum
Read more (PubMed: 24748391) »
Kasioulis I et al. Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis. Mol Biol Cell25:1216-33 (2014).
Read more (PubMed: 24554764) »