• Product name
    Anti-Methylated Lysine antibody
    See all Methylated Lysine primary antibodies
  • Description
    Rabbit polyclonal to Methylated Lysine
  • Specificity
    ab76118 recognizes proteins with trimethylation on lysine residues (N-epsilon). It does not cross-react with acetylated proteins and mono and dimethylated proteins.
  • Tested applications
    Suitable for: IHC-Fr, WB, IP, ICC/IFmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    Methylated KLH conjugates



Our Abpromise guarantee covers the use of ab76118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/1000.
WB 1/1000.
IP Use at 5 µg/mg of lysate.
ICC/IF 1/100.


  • Relevance
    Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
  • Alternative names
    • Dimethylysine antibody
    • Methyl lysine antibody
    • N epsilon dimethyl lysine antibody
    • N epsilon mono methyllysine antibody
    • N epsilon trimethyl lysine antibody
    • N(epsilon) trimethyl lysine antibody
    • Trimethyl lysine antibody
    see all

Anti-Methylated Lysine antibody images

  • ab76118 staining Methylated Lysine in Human Breast tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Western blot analysis of protein trimethylated from human melanoma cell (MMRU) labeling Methylated Lysine with ab76118.
    Left: without competitive inhibition with N-(epsilon) Tri-methyl lysine
    Right: competitive inhibition with N-(epsilon) Tri-methyl lysine (50 μg/ml).

References for Anti-Methylated Lysine antibody (ab76118)

This product has been referenced in:
  • Alban C  et al. Uncovering the protein lysine and arginine methylation network in Arabidopsis chloroplasts. PLoS One 9:e95512 (2014). WB ; Arabidopsis thaliana, Spinacia oleracea, Pisum sativum . Read more (PubMed: 24748391) »
  • Kasioulis I  et al. Kdm3a lysine demethylase is an Hsp90 client required for cytoskeletal rearrangements during spermatogenesis. Mol Biol Cell 25:1216-33 (2014). Read more (PubMed: 24554764) »

See all 5 Publications for this product

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