Anti-Methylated Lysine antibody (Biotin) (ab53524)

Overview

  • Product nameAnti-Methylated Lysine antibody (Biotin)
    See all Methylated Lysine primary antibodies
  • Description
    Rabbit polyclonal to Methylated Lysine (Biotin)
  • ConjugationBiotin
  • Specificityab53524 recognises proteins methylated on lysine residues (mono and di-methylysine). It does not cross-react with acetylated proteins. Cross-reactivity with tri-methyllysine has not been tested.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Species independent
  • Immunogen

    A Methylated KLH conjugated protein[s]

  • General notes

    Purified antibody was conjugated to d-Biotin via acylation. Acylation was with NHS-actvated dBiotin, Biotin/Ab molar ratio: 10:1

Properties

Applications

Our Abpromise guarantee covers the use of ab53524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/1000.
    WB: 1/1000.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceMany proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains.
    • Alternative names
      • Dimethylysine antibody
      • Methyl lysine antibody
      • N epsilon dimethyl lysine antibody
      • N epsilon mono methyllysine antibody
      • N epsilon trimethyl lysine antibody
      • N(epsilon) trimethyl lysine antibody
      • Trimethyl lysine antibody
      see all

    References for Anti-Methylated Lysine antibody (Biotin) (ab53524)

    ab53524 has not yet been referenced specifically in any publications.

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