HRP Anti-Methylated Lysine (di methyl , mono methyl ) antibody (ab23367)
Key features and details
- HRP Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl )
- Suitable for: ELISA, WB
- Reacts with: Species independent
- Conjugation: HRP
- Isotype: IgG
Overview
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Product name
HRP Anti-Methylated Lysine (di methyl , mono methyl ) antibody
See all Methylated Lysine primary antibodies -
Description
HRP Rabbit polyclonal to Methylated Lysine (di methyl , mono methyl ) -
Host species
Rabbit -
Conjugation
HRP -
Specificity
Recognises proteins methylated on lysine residues (mono and dimethy-lysine). Does not cross react with acetylated proteins. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Methylated KLH conjugates.
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General notes
This antibody can be used for detection, quantification and isolation of proteins containing methylated lysine residues. Direct labelling of this primary antibody with HRP means that secondary antibody conjugates are not required.
Reductive amination with HRP to antibody molar ratio of 2:1.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6
Constituents: 0.268% PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Affinity purified using methyl-lysine and di-methyl-lysine on agarose -
Primary antibody notes
This antibody can be used for detection, quantification and isolation of proteins containing methylated lysine residues. Direct labelling of this primary antibody with HRP means that secondary antibody conjugates are not required. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23367 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent dilution.
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|
WB |
Use at an assay dependent dilution.
|
Notes |
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ELISA
Use at an assay dependent dilution. |
WB
Use at an assay dependent dilution. |
Target
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Relevance
Many proteins are post translationally modified. Modifications such as phosphorylation, glycosylation, ubiquitination and methylation have been shown to play an important role in the development, physiology and disease of animals and plants. Histone lysine methylation is regarded as a very stable modification with important functions in epigenetic gene control and for organizing chromatin domains. -
Alternative names
- Dimethylysine antibody
- Methyl lysine antibody
- N epsilon dimethyl lysine antibody
see all
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab23367 has been referenced in 1 publication.
- Schuhmacher MK et al. The Legionella pneumophila Methyltransferase RomA Methylates Also Non-histone Proteins during Infection. J Mol Biol 430:1912-1925 (2018). PubMed: 29733858