Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)

Overview

  • Product nameAnti-Methylmalonyl Coenzyme A mutase antibody
    See all Methylmalonyl Coenzyme A mutase primary antibodies
  • Description
    Mouse polyclonal to Methylmalonyl Coenzyme A mutase
  • Tested applicationsSuitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Full length Methylmalonyl Coenzyme A mutase protein (Human)

  • Positive control
    • Methylmalonyl Coenzyme A mutase transfected 293T cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab67869 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 10 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately 83 kDa (predicted molecular weight: 83 kDa).
IHC-P Use at an assay dependent dilution. PubMed: 19699272

Target

  • FunctionInvolved in the degradation of several amino acids, odd-chain fatty acids and cholesterol via propionyl-CoA to the tricarboxylic acid cycle. MCM has different functions in other species.
  • Involvement in diseaseDefects in MUT are the cause of methylmalonic aciduria type mut (MMAM) [MIM:251000]. MMAM is an often fatal disorder of organic acid metabolism. Common clinical features include lethargy, vomiting, failure to thrive, hypotonia, neurological deficit and early death. Two forms of the disease are distinguished by the presence (mut-) or absence (mut0) of residual enzyme activity. Mut0 patients have more severe neurological manifestations of the disease than do MUT- patients. MMAM is unresponsive to vitamin B12 therapy.
  • Sequence similaritiesBelongs to the methylmalonyl-CoA mutase family.
    Contains 1 B12-binding domain.
  • Cellular localizationMitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • MCM antibody
    • Methylmalonyl CoA isomerase antibody
    • Methylmalonyl CoA mutase mitochondrial antibody
    • Methylmalonyl Coenzyme A mutase antibody
    • Methylmalonyl-CoA isomerase antibody
    • Methylmalonyl-CoA mutase antibody
    • mitochondrial antibody
    • Mut antibody
    • MUTA_HUMAN antibody
    see all

Anti-Methylmalonyl Coenzyme A mutase antibody images

  • Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution + human liver tissue lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • All lanes : Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869) at 1/500 dilution

    Lane 1 : Methylmalonyl Coenzyme A mutase transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 83 kDa
    Observed band size : 83 kDa
  • ICC/IF image of ab67869 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab67869, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96879, DyLight® 488 goat anti-mouse IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-Methylmalonyl Coenzyme A mutase antibody (ab67869)

This product has been referenced in:
  • Ruppert T  et al. Molecular and biochemical alterations in tubular epithelial cells of patients with isolated methylmalonic aciduria. Hum Mol Genet 24:7049-59 (2015). Read more (PubMed: 26420839) »
  • Ballhausen D  et al. Evidence for catabolic pathway of propionate metabolism in CNS: expression pattern of methylmalonyl-CoA mutase and propionyl-CoA carboxylase alpha-subunit in developing and adult rat brain. Neuroscience 164:578-87 (2009). WB, IHC-P ; Rat . Read more (PubMed: 19699272) »

See all 2 Publications for this product

Product Wall

Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from this antibody.

I would like to reassure you that this antibody is tested and covered by our 6 month guarantee for ICC...

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"