Anti-METTL11A antibody (ab72660)
Key features and details
- Mouse polyclonal to METTL11A
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-METTL11A antibody -
Description
Mouse polyclonal to METTL11A -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human METTL11A. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- Human liver lysate METTL11A transfected 293T lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa). |
Target
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Function
Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala, Pro or Ser residue in the [Ala/Pro/Ser]-Pro-Lys motif. Mediates the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation. -
Sequence similarities
Belongs to the methyltransferase superfamily. NTM1 family. -
Cellular localization
Nucleus. Predominantly nuclear. - Information by UniProt
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Database links
- Entrez Gene: 28989 Human
- Omim: 613560 Human
- SwissProt: Q9BV86 Human
- Unigene: 522433 Human
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Alternative names
- AD 003 antibody
- Alpha N-terminal protein methyltransferase 1A antibody
- C9orf32 antibody
see all
Images
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Anti-METTL11A antibody (ab72660) at 1/500 dilution + Human liver lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: 25 kDa -
All lanes : Anti-METTL11A antibody (ab72660) at 1/500 dilution
Lane 1 : METTL11A transfected 293T lysate
Lane 2 : Non-transfected 293T lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 25 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (2)
ab72660 has been referenced in 2 publications.
- Bade D et al. Modulation of N-terminal methyltransferase 1 by an N6-methyladenosine-based epitranscriptomic mechanism. Biochem Biophys Res Commun 546:54-58 (2021). PubMed: 33561748
- Park JH et al. Disruption of nucleocytoplasmic trafficking as a cellular senescence driver. Exp Mol Med 53:1092-1108 (2021). PubMed: 34188179