Overview

  • Product name
  • Description
    Mouse polyclonal to METTL11A
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, Human METTL11A (NP_054783).

  • Positive control
    • Human liver lysate METTL11A transfected 293T lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab72660 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).

Target

  • Function
    Alpha-N-methyltransferase that methylates the N-terminus of target proteins containing the N-terminal motif [Ala/Pro/Ser]-Pro-Lys when the initiator Met is cleaved. Specifically catalyzes mono-, di- or tri-methylation of exposed alpha-amino group of Ala, Pro or Ser residue in the [Ala/Pro/Ser]-Pro-Lys motif. Mediates the N-terminal methylation of KLHL31, MYL2, MYL3, RB1, RCC1, RPL23A and SET. Required during mitosis for normal bipolar spindle formation and chromosome segregation.
  • Sequence similarities
    Belongs to the methyltransferase superfamily. NTM1 family.
  • Cellular localization
    Nucleus. Predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • AD 003 antibody
    • Alpha N-terminal protein methyltransferase 1A antibody
    • C9orf32 antibody
    • Chromosome 9 open reading frame 32 antibody
    • Methyltransferase like 11A antibody
    • Methyltransferase-like protein 11A antibody
    • Mettl11a antibody
    • N-terminal RCC1 methyltransferase antibody
    • NRMT antibody
    • NTM1A antibody
    • NTM1A_HUMAN antibody
    • NTMT1 antibody
    • X-Pro-Lys N-terminal protein methyltransferase 1A antibody
    see all

Images

  • Anti-METTL11A antibody (ab72660) at 1/500 dilution + Human liver lysate at 25 µg

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 25 kDa
  • All lanes : Anti-METTL11A antibody (ab72660) at 1/500 dilution

    Lane 1 : METTL11A transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 25 kDa
    Observed band size : 24 kDa (why is the actual band size different from the predicted?)

References

ab72660 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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