Overview

  • Product nameAnti-METTL14 antibody
    See all METTL14 primary antibodies
  • Description
    Rabbit polyclonal to METTL14
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Cow, Dog, Pig, Zebrafish
  • Immunogen

    Synthetic peptide corresponding to a region within N terminal amino acids 35-84 of Human METTL14 (NP_066012).

  • Positive control
    • NCI-H226 cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab98166 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionN6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization.
  • Sequence similaritiesBelongs to the MT-A70-like family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • KIAA1627 antibody
    • MET14_HUMAN antibody
    • Methyltransferase like 14 antibody
    • Methyltransferase-like protein 14 antibody
    • METTL14 antibody
    • N6-adenosine-methyltransferase subunit METTL14 antibody
    • OTTHUMP00000219837 antibody
    see all

Anti-METTL14 antibody images

  • Anti-METTL14 antibody (ab98166) at 1 µg/ml (in 5% skim milk / PBS buffer) + NCI-H226 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 52 kDa

References for Anti-METTL14 antibody (ab98166)

ab98166 has not yet been referenced specifically in any publications.

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (human embryonic stem cells)
Specification human embryonic stem cells
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 26 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human embryonic stem cells)
Gel Running Conditions Reduced Denaturing (4-20%)
Loading amount 30 µg
Specification human embryonic stem cells
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Username

Abcam user community

Verified customer

Submitted Jan 21 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293)
Loading amount 20 µg
Specification HEK293
Treatment siRNA towards METTL14 (Dharmacon)
Gel Running Conditions Non-reduced Non-Denaturing (Native) (12% gel)
Blocking step Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Ms. Samantha Johnston

Verified customer

Submitted Feb 24 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"