Anti-METTL14 antibody [CL4252] (ab220030)
Key features and details
- Mouse monoclonal [CL4252] to METTL14
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-METTL14 antibody [CL4252]
See all METTL14 primary antibodies -
Description
Mouse monoclonal [CL4252] to METTL14 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Chicken, Cow, Orangutan -
Immunogen
Recombinant fragment (proprietary-tag) corresponding to Human METTL14 aa 1-100.
Database link: Q9HCE5 -
Positive control
- Human testis, Human endometrium, Human prostate, Human cervix, Human cerebral cortex and Human duodenum tissues; A431 cell line lysate. ICC/IF: RH-30 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL4252 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220030 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
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WB |
1/500 - 1/1000. Predicted molecular weight: 52 kDa.
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IHC-P | (2) |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 2 - 10 µg/ml. |
WB
1/500 - 1/1000. Predicted molecular weight: 52 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
N6-methyltransferase that methylates adenosine residues of some mRNAs and acts as a regulator of the circadian clock and differentiation of embryonic stem cells. N6-methyladenosine (m6A), which takes place at the 5'-[AG]GAC-3' consensus sites of some mRNAs, plays a role in the efficiency of mRNA splicing, processing and mRNA stability (PubMed:24316715, PubMed:24407421, PubMed:25719671). M6A regulates the length of the circadian clock: acts as a early pace-setter in the circadian loop. M6A also acts as a regulator of mRNA stability: in embryonic stem cells (ESCs), m6A methylation of mRNAs encoding key naive pluripotency-promoting transcripts results in transcript destabilization. -
Sequence similarities
Belongs to the MT-A70-like family. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 57721 Human
- Entrez Gene: 210529 Mouse
- Omim: 616504 Human
- SwissProt: Q5ZK35 Chicken
- SwissProt: A4IFD8 Cow
- SwissProt: Q9HCE5 Human
- SwissProt: Q3UIK4 Mouse
- SwissProt: Q5R5N4 Orangutan
see all -
Alternative names
- KIAA1627 antibody
- MET14_HUMAN antibody
- Methyltransferase like 14 antibody
see all
Images
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Anti-METTL14 antibody [CL4252] (ab220030) at 1/500 dilution + A431 cell line lysate
Predicted band size: 52 kDa -
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human duodenum tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in glandular cells and in the connective tissue.
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Immunocytochemistry/ Immunofluorescence anaysis of RH-30 cells labeling METTL14 with ab220030 at 2-10 µg/mL. Cells were fixed with PFA and permeabilised with Triton X-100. The data is showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cerebral cortex tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing moderate nuclear positivity in neurons.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human cervix tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in squamous ephitelium and connective tissue cells.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human prostate tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in glandular cells and in smooth muscle.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human endometrium tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear immunoreactivity in glandular cells and in stroma.
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Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human testis tissue labeling METTL14 with ab220030 at 1/1000 dilution, showing strong nuclear positivity in cells in seminiferous tubes.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (25)
ab220030 has been referenced in 25 publications.
- Zheng Y et al. LncNAP1L6 activates MMP pathway by stabilizing the m6A-modified NAP1L2 to promote malignant progression in prostate cancer. Cancer Gene Ther 30:209-218 (2023). PubMed: 36195720
- Yi D et al. Deciphering the map of METTL14-mediated lncRNA m6A modification at the transcriptome-wide level in breast cancer. J Clin Lab Anal 36:e24754 (2022). PubMed: 36336884
- Luo M et al. PHLDB2 Mediates Cetuximab Resistance via Interacting With EGFR in Latent Metastasis of Colorectal Cancer. Cell Mol Gastroenterol Hepatol 13:1223-1242 (2022). PubMed: 34952201
- Li Y et al. Downregulation of Methyltransferase-Like 14 Promotes Ovarian Cancer Cell Proliferation Through Stabilizing TROAP mRNA. Front Oncol 12:824258 (2022). PubMed: 35251990
- Lin JX et al. m6A methylation mediates LHPP acetylation as a tumour aerobic glycolysis suppressor to improve the prognosis of gastric cancer. Cell Death Dis 13:463 (2022). PubMed: 35568711