Overview

  • Product name
    Anti-METTL21B antibody
  • Description
    Rabbit polyclonal to METTL21B
  • Tested applications
    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to amino acids 158-226 of Human METTL21B (UniProt ID: Q96AZ1).

  • Positive control
    • Human testis tissue
  • General notes
    The antibody solution should be gently mixed before use.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab150900 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

Target

  • Function
    Protein-lysine methyltransferase.
  • Sequence similarities
    Belongs to the methyltransferase superfamily. METTL21 family.
  • Cellular localization
    Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • FAM119B antibody
    • Hepatocellular carcinoma-associated antigen 557a antibody
    • Methyltransferase like 21B antibody
    • Methyltransferase-like protein 21B antibody
    • mettl21b antibody
    • MT21B_HUMAN antibody
    • Protein-lysine methyltransferase METTL21B antibody
    see all

Images

  • Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus but not nucleoli. Recommended concentration of ab150900 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling METTL21B with ab150900 at 1/200 dilution.

References

ab150900 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab150900.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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