Overview

  • Product nameAnti-METTL21D antibody
  • Description
    Rabbit polyclonal to METTL21D
  • Tested applicationsSuitable for: ICC/IF, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment, corresponding to amino acids 3-143 of Human METTL21D (Q9H867-1).

  • Positive control
    • Human breast tissue.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferpH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: 59% PBS, 40% Glycerol
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab121127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 4 µg/ml.

Recommend PFA Fixation and Triton X-100 treatment

IHC-P 1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. (retrieval buffer pH 6).

Target

Anti-METTL21D antibody images

  • Immunofluorescent staining of Human cell line U-2 OS shows positivity in nucleus but not nucleoli and cytoplasm. Recommended concentration of ab121127 1-4 µg/ml. Cells treated with PFA/Triton X-100.
  • ab121127 at 1/125 dilution staining METTL21D in Paraffin-emebedded Human breast tissue by Immunohistochemistry.

References for Anti-METTL21D antibody (ab121127)

ab121127 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"