Overview

  • Product name
  • Description
    Rabbit polyclonal to METTL7A
  • Tested applications
    Suitable for: WB, ELISA, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide from the N terminal region of human METTL7A, conjugated to KLH.

  • Positive control
    • 293 cell lysates transiently transfected with the METTL7A gene. Human hepatocarcinoma tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    ab62969 was purified through a protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab62969 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/50 - 1/100. Detects a band of approximately 25 kDa (predicted molecular weight: 28 kDa).
ELISA 1/1000.
IHC-P 1/10 - 1/50.

Target

  • Function
    Probable methyltransferase.
  • Sequence similarities
    Belongs to the methyltransferase superfamily.
  • Cellular localization
    Lipid droplet. Endoplasmic reticulum. Membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • AAM B antibody
    • AAMB antibody
    • DKFZP586A0522 antibody
    • MET7A_HUMAN antibody
    • Methyltransferase like 7A antibody
    • Methyltransferase-like protein 7A antibody
    • METTL 7A antibody
    • METTL7A antibody
    • METTL7A methlytransferase like 7A antibody
    • Protein AAM-B antibody
    • Protein AAMB antibody
    see all

Images

  • All lanes : Anti-METTL7A antibody (ab62969) at 1/50 dilution

    Lane 1 : 293 cell lysates nontransfected
    Lane 2 : 293 cell lysates transiently transfected with the METTL7A gene

    Lysates/proteins at 2 µg per lane.


    Predicted band size : 28 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • ab62969, at 1/10 dilution, staining METTL7A in formalin-fixed, paraffin-embedded human hepatocarcinoma tissue by immunohistochemistry. ab62969 was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

References

ab62969 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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