• Product name
  • Description
    Rabbit polyclonal to MFF
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Rat, Human, Zebrafish
    Predicted to work with: Mouse, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog
  • Immunogen

    Synthetic peptide corresponding to a region within C terminal amino acids 287-336 (LTVVDAASLR RQIIKLNRRL QLLEEENKER AKREMVMYSI TVAFWLLNSW) of Human MFF (NP_064579).

  • Positive control
    • Fetal muscle lysate. This antibody gave a positive result in IF in the following Formaldehyde fixed cell line: A549



Our Abpromise guarantee covers the use of ab81127 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 38 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.


Anti-MFF antibody images

  • Anti-MFF antibody (ab81127) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µg

    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 38 kDa
    Observed band size : 38 kDa
    Gel concentration 12%
  • All lanes : Anti-MFF antibody (ab81127) at 1 µg/ml

    Lane 1 : Marker
    Lane 2 : Zebrafish brain homogenate at 20 µg
    Lane 3 : Zebrafish heart homogenate at 20 µg
    Lane 4 : Zebrafish liver homogenate at 20 µg

    Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 38 kDa
    Observed band size : 37 kDa (why is the actual band size different from the predicted?)

    Exposure time : 3 minutes
  • ab81127 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81127 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-MFF antibody (ab81127)

This product has been referenced in:
  • Bertholet AM  et al. OPA1 loss of function affects in vitro neuronal maturation. Brain 136:1518-33 (2013). Rat . Read more (PubMed: 23543485) »
  • Guido C  et al. Mitochondrial fission induces glycolytic reprogramming in cancer-associated myofibroblasts, driving stromal lactate production, and early tumor growth. Oncotarget 3:798-810 (2012). WB ; Human . Read more (PubMed: 22878233) »

See all 2 Publications for this product

Product Wall

Western blot
Mouse Cell lysate - whole cell (p38 mouse melanoma cell line)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Mff KD siRNA
p38 mouse melanoma cell line
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Ms. Carmen Adriaens

Verified customer

Submitted Aug 07 2015

The immunogens differ, though both are closer to the C terminus than the N terminus. The immunogen for ab81127 is derived from within (but is not identical to) the range given on the datasheet, amino acids 287-336 of human MFF. The immunogen for a...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Western blot
Rat Cell lysate - whole cell (Neurones primaires corticaux)
Loading amount
1.2e+006 cells
Neurones primaires corticaux
Gel Running Conditions
Reduced Denaturing (gel 15%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mrs. Marlene Daloyau

Verified customer

Submitted Oct 16 2012

Code de remise pour le test de ab74257: *******
Code de remise pour le test de ab81127 : ****** (ci-joint l'alignement de la région aa 30-100 avec la protéine de rat)
Code de remise pour le test de ab101908 : *******


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Thank you for contacting us.

Regarding antibodies ab79397 and ab32577, they both have been tested in IHC with paraffin embedded sections in the species stated on the datasheet. We don’t have preliminary data using them with frozen secti...

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