The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 62 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use at an assay dependent concentration.
ELISA titre using peptide based assay, 1:62500.
RelevanceMembrane frizzled-related protein, MFRP, may have a role in eye development and may be necessary for photoreceptor maintenance. Mutations of membrane-type frizzled-related protein are associated with inherited retinal degenerations including syndromic forms of microphthalmos/retinal degeneration, a rare autosomal recessive disorder of eye development characterized by extreme hyperopia and small functional eyes,posterior microphthalmia with retinitis pigmentosa, foveoschisis, and optic disk drusen.
Cellular localizationCell Membrane; single-pass type II membrane protein