Overview

  • Product name
  • Description
    Rabbit polyclonal to MGA
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Immungen corresponds to a region within amino acids 2450-2500 of Human MGA (NP_001074010.2)

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      MGA contains 1 basic helix-loop-helix (bHLH) domain and is a possible transcription factor.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • MAD5 antibody
      • MAX dimerization protein 5 antibody
      • MAX gene associated antibody
      • MAX gene associated protein antibody
      • Max interacting protein antibody
      • MGAM antibody
      • MXD5 antibody
      see all

    Anti-MGA antibody images

    • Detection of Human MGA by Immunoprecipitation in 1mg HeLa whole cell lysate (20% of IP loaded) using ab101269 at 3µg/mg of lysate. A different anti MGA antibody was used for subsequent WB detection using Chemiluminescence with an exposure time of 10 seconds.

    References for Anti-MGA antibody (ab101269)

    ab101269 has not yet been referenced specifically in any publications.

    Product Wall

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Epiblast)
    Specification
    Epiblast
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Hydrogen Peroxide, DMSO
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    Mr. Andy Washkowitz

    Verified customer

    Submitted Jan 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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