Overview

  • Product name
  • Description
    Rabbit polyclonal to MGA
  • Tested applications
    Suitable for: IPmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Immungen corresponds to a region within amino acids 2450-2500 of Human MGA (NP_001074010.2)

  • Positive control
    • HeLa whole cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate buffer, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab101269 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at 2-5 µg/mg of lysate.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      MGA contains 1 basic helix-loop-helix (bHLH) domain and is a possible transcription factor.
    • Cellular localization
      Nuclear
    • Database links
    • Alternative names
      • MAD5 antibody
      • MAX dimerization protein 5 antibody
      • MAX gene associated antibody
      • MAX gene associated protein antibody
      • Max interacting protein antibody
      • MGAM antibody
      • MXD5 antibody
      see all

    Images

    • Detection of Human MGA by Immunoprecipitation in 1mg HeLa whole cell lysate (20% of IP loaded) using ab101269 at 3µg/mg of lysate. A different anti MGA antibody was used for subsequent WB detection using Chemiluminescence with an exposure time of 10 seconds.

    References

    ab101269 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry
    Sample
    Mouse Cell (Epiblast)
    Specification
    Epiblast
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - Hydrogen Peroxide, DMSO
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
    Username

    Mr. Andy Washkowitz

    Verified customer

    Submitted Jan 04 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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