Overview

  • Product name
  • Description
    Goat polyclonal to MGAT1
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Rabbit
  • Immunogen

    Synthetic peptide:

    C-QVEKVRTNDR

    , corresponding to internal sequence amino acids 369-378 of Human MGAT1 (NP_002397.2).

  • Positive control
    • MGAT1 transfected HEK293 lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.02% Sodium Azide
    Constituents: 0.5% BSA, Tris buffered saline, pH 7.3
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab109552 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
  • Tissue specificity
    Appears to be present in all tissues.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 13 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
    • GGNT1 antibody
    • GLCNAC TI antibody
    • GlcNAc-T I antibody
    • GLCT1 antibody
    • GLYT1 antibody
    • GNT 1 antibody
    • GNT-I antibody
    • Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase antibody
    • MGAT antibody
    • MGAT1 antibody
    • MGAT1_HUMAN antibody
    • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I antibody
    see all

Images

  • All lanes : Anti-MGAT1 antibody (ab109552) at 1 µg/ml

    Lane 1 : HEK293 lysate in RIPA buffer, overexpressing Human MGAT1 with C terminal MYC tag
    Lane 2 : Mock transfected HEK293 lysate

    Lysates/proteins at 10 µg per lane.

    Developed using the ECL technique

    Predicted band size : 50 kDa
    Observed band size : 55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 hour

References

ab109552 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab109552.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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