Overview

  • Product name
  • Description
    Mouse polyclonal to MGAT1
  • Host species
    Mouse
  • Tested applications
    Suitable for: WB, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit
  • Immunogen

    Full length protein, corresponding to amino acids 1-445 of Human MGAT1 (NP_002397.1).

  • Positive control
    • MGAT1 transfected 293T cell line lysate; HeLa cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • Storage buffer
    pH: 7.20
    Constituent: 99% PBS
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab167365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 51 kDa.
ICC/IF Use a concentration of 10 µg/ml.

Target

  • Function
    Initiates complex N-linked carbohydrate formation. Essential for the conversion of high-mannose to hybrid and complex N-glycans.
  • Tissue specificity
    Appears to be present in all tissues.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 13 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
    • Alpha-1 antibody
    • Alpha-1,3-mannosyl-glycoprotein 2-beta-N-acetylglucosaminyltransferase antibody
    • GGNT1 antibody
    • GLCNAC TI antibody
    • GlcNAc-T I antibody
    • GLCT1 antibody
    • GLYT1 antibody
    • GNT 1 antibody
    • GNT-I antibody
    • Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase antibody
    • MGAT antibody
    • MGAT1 antibody
    • MGAT1_HUMAN antibody
    • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase I antibody
    see all

Images

  • All lanes : Anti-MGAT1 antibody (ab167365) at 1 µg/ml

    Lane 1 : MGAT1 transfected 293T cell line lysate
    Lane 2 : Non-transfected 293T cell line lysate

    Lysates/proteins at 15 µl per lane.

    Secondary
    All lanes : Goat anti-Mouse IgG HRP at 1/2500 dilution

    Predicted band size: 51 kDa

  • Immunofluorescence analysis of HeLa cells labeling MGAT1 using ab167365 at 10 µg/ml.

References

This product has been referenced in:
  • Petrosyan A  et al. Keratin 1 plays a critical role in golgi localization of core 2 N-acetylglucosaminyltransferase M via interaction with its cytoplasmic tail. J Biol Chem 290:6256-69 (2015). Read more (PubMed: 25605727) »

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Mice hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Mice hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted May 24 2018

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Jan 19 2016

Application
Western blot
Sample
Human Cell lysate - whole cell (HepG2 cells)
Gel Running Conditions
Reduced Denaturing (15% SDS-PAGE)
Loading amount
35 µg
Specification
HepG2 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

Submitted Nov 19 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Human Cell (A549)
Specification
A549
Permeabilization
No
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Sep 30 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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