Overview

  • Product name
  • Description
    Rabbit polyclonal to MGAT3
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length protein, corresponding to amino acids 1-533 of Human MGAT3 (AAH75025.1).

  • Positive control
    • Human placenta tissue lysate; MGAT3 transfected 293T cell lysate

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103427 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 61 kDa.

Target

  • Function
    It is involved in the regulation of the biosynthesis and biological function of glycoprotein oligosaccharides. Catalyzes the addition of N-acetylglucosamine in beta 1-4 linkage to the beta-linked mannose of the trimannosyl core of N-linked sugar chains. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
  • Pathway
    Protein modification; protein glycosylation.
  • Sequence similarities
    Belongs to the glycosyltransferase 17 family.
  • Cellular localization
    Golgi apparatus membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 4-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase antibody
    • Beta 1 4 mannosyl glycoprotein 4 beta N acetylglucosaminyltransferase antibody
    • Beta-1 antibody
    • FLJ43371 antibody
    • GlcNAc T III antibody
    • GlcNAc-T III antibody
    • GNT 3 antibody
    • GNT III antibody
    • GNT-III antibody
    • GNT3 antibody
    • Mannosyl (beta 1 4 ) glycoprotein beta 1 4 N acetylglucosaminyltransferase antibody
    • MGAT 3 antibody
    • MGAT3 antibody
    • MGAT3_HUMAN antibody
    • MGC141943 antibody
    • MGC142278 antibody
    • N acetylglucosaminyltransferase III antibody
    • N glycosyl oligosaccharide glycoprotein N acetylglucosaminyltransferase III antibody
    • N-acetylglucosaminyltransferase III antibody
    • N-glycosyl-oligosaccharide-glycoprotein N-acetylglucosaminyltransferase III antibody
    • OTTHUMP00000042207 antibody
    see all

Images

  • Anti-MGAT3 antibody (ab103427) at 1/500 dilution + Human placenta lysate at 50 µg

    Predicted band size : 61 kDa
  • All lanes : Anti-MGAT3 antibody (ab103427) at 1/500 dilution

    Lane 1 : MGAT3 transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 61 kDa

References

ab103427 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Application
Western blot
Sample
Rat Cell lysate - whole cell (Rat hepatocytes)
Gel Running Conditions
Reduced Denaturing (4-15% SDS PAGE)
Loading amount
25 µg
Specification
Rat hepatocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Dr. Armen Petrosyan

Verified customer

Submitted Nov 13 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (LNCaP - prostate cancer cells)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
LNCaP - prostate cancer cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

Submitted Oct 11 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (Rat hepatocytes)
Permeabilization
Yes - 0.2% Triton X-100 in PBS
Specification
Rat hepatocytes
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

Submitted Sep 26 2017

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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