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Overview
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Product nameAnti-MGAT4B antibody
See all MGAT4B primary antibodies -
DescriptionRabbit polyclonal to MGAT4B
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Human
Predicted to work with: Mouse
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Immunogen
Recombinant fragment corresponding to Human MGAT4B aa 82-240. (BC009464)
Sequence:IAETDSQYTSAVTENIKALFPTEIHSGLLEVISPSPHFYPDFSRLRESFG DPKERVRWRTKQNLDYCFLMMYAQSKGIYYVQLEDDIVAKPNYLSTMKNF ALQQPSEDWMILEFSQLGFIGKMFKSLDLSLIVEFILMFYRDKPIDWLLD HILWVKVCN
Database link: Q9UQ53 -
Positive control
- HepG2 & Human fetal kidney lysates, Human kidney tissue
Properties
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FormLyophilised:Reconstitute in 200ul sterile H2O.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferpH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 98% PBS, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
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Related Products
Applications
Our Abpromise guarantee covers the use of ab177206 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | 1/100 - 1/500. | |
| WB | 1/200 - 1/1000. Predicted molecular weight: 63 kDa. |
Target
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FunctionGlycosyltransferase that participates in the transfer of N-acetylglucosamine (GlcNAc) to the core mannose residues of N-linked glycans. Catalyzes the formation of the GlcNAcbeta1-4 branch on the GlcNAcbeta1-2Manalpha1-3 arm of the core structure of N-linked glycans. Essential for the production of tri- and tetra-antennary N-linked sugar chains. Has lower affinities for donors or acceptors than MGAT4A, suggesting that, under physiological conditions, it is not the main contributor in N-glycan biosynthesis.
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Tissue specificityWidely expressed. Strongly overexpressed in pancreatic cancer.
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PathwayProtein modification; protein glycosylation.
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Sequence similaritiesBelongs to the glycosyltransferase 54 family.
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Cellular localizationGolgi apparatus membrane.
- Information by UniProt
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Database links
- Entrez Gene: 11282 Human
- Entrez Gene: 103534 Mouse
- Omim: 604561 Human
- SwissProt: Q9UQ53 Human
- SwissProt: Q812F8 Mouse
- Unigene: 567419 Human
- Unigene: 86759 Mouse
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Alternative names
- 3-D-mannoside beta-1 antibody
- 3-mannosyl-glycoprotein 4-beta-N-acetylglucosaminyltransferase B antibody
- 4-N-acetylglucosaminyltransferase IVb antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MGAT4B antibody (ab177206)Immunohistochemical analysis of formalin-fixed paraffin-embedded Human kidney tissue labeling MGAT4B with ab177206 at 1/100 dilution.
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Western blot - Anti-MGAT4B antibody (ab177206)All lanes : Anti-MGAT4B antibody (ab177206) at 1/500 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : Human fetal kidney lysate
Predicted band size: 63 kDa
References
ab177206 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"