Human Predicted to work with:
Mouse, Rat, Hamster
Recombinant fragment: LAEPGQSCKQ VCQESQLICE PSFFQHLNKD KDMLKYKVTC QSSELAKDIL VPSFDPKNK HCVFQGDLLL FSCAGAHPRH QRVCPCRDFI KGQVALCKD, corresponding to amino acids 642 - 739 of Human MGAT5 (NP_002401) with a proprietary tag
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 85 kDa.
Use at an assay dependent concentration.
Use 0.1-1µg for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
FunctionCatalyzes the addition of N-acetylglucosamine in beta 1-6 linkage to the alpha-linked mannose of biantennary N-linked oligosaccharides. It is one of the most important enzymes involved in the regulation of the biosynthesis of glycoprotein oligosaccharides.
PathwayProtein modification; protein glycosylation.
Sequence similaritiesBelongs to the glycosyltransferase 18 family.
Overlay histogram showing Jurkat cells stained with ab87977 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab87977, 1μg/1x106 cells) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-mouse IgG (H&L) (ab150113) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Western blot - MGAT5 antibody (ab87977)
Anti-MGAT5 antibody (ab87977) at 1 µg/ml + recombinant protein fragment with a proprietary tag (the immunogen) at 0.1 µg
Secondary Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution