Overview

  • Product nameAnti-MGEA5 antibody
    See all MGEA5 primary antibodies
  • Description
    Mouse polyclonal to MGEA5
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length human MGEA5 (NP_036347.1).

  • Positive control
    • MGEA5 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 103 kDa.

Target

  • FunctionIsoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
    Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
  • Tissue specificityUbiquitous. Shows highest expression in the brain, placenta and pancreas.
  • Sequence similaritiesBelongs to the glycosyl hydrolase 84 family.
  • Post-translational
    modifications
    Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
  • Cellular localizationCytoplasm and Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • Beta-hexosaminidase antibody
    • Beta-N-acetylglucosaminidase antibody
    • Beta-N-acetylhexosaminidase antibody
    • Bifunctional protein NCOAT antibody
    • HEXC antibody
    • Hyaluronidase in meningioma antibody
    • KIAA0679 antibody
    • MEA5 antibody
    • Meningioma expressed antigen 5 antibody
    • Meningioma-expressed antigen 5 antibody
    • MGEA 5 antibody
    • Mgea5 antibody
    • N-acetyl-beta-D-glucosaminidase antibody
    • N-acetyl-beta-glucosaminidase antibody
    • NCOAT antibody
    • Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody
    • Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody
    • OGA antibody
    • OGA_HUMAN antibody
    • Protein O-GlcNAcase antibody
    see all

Anti-MGEA5 antibody images

  • Anti-MGEA5 antibody (ab68522) at 1/2000 dilution + 293T Cell Lysate at 10 µg with 3% Milk

    Secondary
    HRP conjugated Rat anti-mouse polyclonal at 1/1000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 103 kDa


    Exposure time : 1 minute

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-MGEA5 antibody (ab68522)

This product has been referenced in:
  • Lynch TP  et al. Critical role of O-Linked ß-N-acetylglucosamine transferase in prostate cancer invasion, angiogenesis, and metastasis. J Biol Chem 287:11070-81 (2012). WB ; Human . Read more (PubMed: 22275356) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (mast cell type)
Gel Running Conditions Non-reduced Non-Denaturing (Native)
Loading amount 10 µg
Specification mast cell type
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Mr. Seong Hoon Kim

Verified customer

Submitted May 24 2016

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing (8%)
Sample Human Cell lysate - whole cell (T lymphocyte)
Specification T lymphocyte
Treatment Jurkat T cells for 24hr (without chemical)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Kyeongah Kim

Verified customer

Submitted Feb 04 2015

Abreviews
Application Western blot
Loading amount 30 µg
Gel Running Conditions Non-reduced Denaturing (8%)
Sample Human Cell lysate - whole cell (T lymphocyte)
Specification T lymphocyte
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 10 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (293T)
Loading amount 10 µg
Specification 293T
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Mar 01 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"