Anti-MGEA5 antibody [EPR7154(B)] (ab124807)

Overview

  • Product nameAnti-MGEA5 antibody [EPR7154(B)]
    See all MGEA5 primary antibodies
  • Description
    Rabbit monoclonal [EPR7154(B)] to MGEA5
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa, BxPC-3, JAR and fetal brain lysates; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

Applications

Our Abpromise guarantee covers the use of ab124807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 102 kDa).Can be blocked with MGEA5 peptide (ab155521).
IP 1/100 - 1/500.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionIsoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
      Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
    • Tissue specificityUbiquitous. Shows highest expression in the brain, placenta and pancreas.
    • Sequence similaritiesBelongs to the glycosyl hydrolase 84 family.
    • Post-translational
      modifications
      Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
    • Cellular localizationCytoplasm and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta-hexosaminidase antibody
      • Beta-N-acetylglucosaminidase antibody
      • Beta-N-acetylhexosaminidase antibody
      • Bifunctional protein NCOAT antibody
      • HEXC antibody
      • Hyaluronidase in meningioma antibody
      • KIAA0679 antibody
      • MEA5 antibody
      • Meningioma expressed antigen 5 antibody
      • Meningioma-expressed antigen 5 antibody
      • MGEA 5 antibody
      • Mgea5 antibody
      • N-acetyl-beta-D-glucosaminidase antibody
      • N-acetyl-beta-glucosaminidase antibody
      • NCOAT antibody
      • Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody
      • Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody
      • OGA antibody
      • OGA_HUMAN antibody
      • Protein O-GlcNAcase antibody
      see all

    Anti-MGEA5 antibody [EPR7154(B)] images

    • All lanes : Anti-MGEA5 antibody [EPR7154(B)] (ab124807) at 1/10000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : JAR lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 102 kDa
      Observed band size : 130 kDa (why is the actual band size different from the predicted?)
    • ab124807, at 1/100 dilution, staining MGEA5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References for Anti-MGEA5 antibody [EPR7154(B)] (ab124807)

    This product has been referenced in:
    • Park HC  et al. Urinary N-acetyl-ß-D glucosaminidase as a surrogate marker for renal function in autosomal dominant polycystic kidney disease: 1 year prospective cohort study. BMC Nephrol 13:93 (2012). IHC-Fr ; Human . Read more (PubMed: 22935351) »

    See 1 Publication for this product

    Product Wall

    As discussed in my previous email, I have now arranged for the immunizing peptide to be available from our catalogue. This can be viewed under the catalogue number ab155521:

    http://www.abcam.com/ab155521

    I hope this information has be...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Mouse Cell lysate - whole cell (Mouse embryonic stem cell)
    Loading amount 20 µg
    Specification Mouse embryonic stem cell
    Gel Running Conditions Reduced Denaturing (4-12%, MOPS buffer)
    Blocking step Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    Submitted May 07 2012

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"