Anti-MGEA5 antibody [EPR7154(B)] (ab124807)


  • Product name
    Anti-MGEA5 antibody [EPR7154(B)]
    See all MGEA5 primary antibodies
  • Description
    Rabbit monoclonal [EPR7154(B)] to MGEA5
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

  • Positive control
    • HeLa, BxPC-3, JAR and fetal brain lysates; Human kidney tissue
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents



Our Abpromise guarantee covers the use of ab124807 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 130 kDa (predicted molecular weight: 102 kDa).Can be blocked with MGEA5 peptide (ab155521).
IP 1/100 - 1/500.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or ICC.
  • Target

    • Function
      Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
      Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
    • Tissue specificity
      Ubiquitous. Shows highest expression in the brain, placenta and pancreas.
    • Sequence similarities
      Belongs to the glycosyl hydrolase 84 family.
    • Post-translational
      Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
    • Cellular localization
      Cytoplasm and Nucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • Beta-hexosaminidase antibody
      • Beta-N-acetylglucosaminidase antibody
      • Beta-N-acetylhexosaminidase antibody
      • Bifunctional protein NCOAT antibody
      • HEXC antibody
      • Hyaluronidase in meningioma antibody
      • KIAA0679 antibody
      • MEA5 antibody
      • Meningioma expressed antigen 5 antibody
      • Meningioma-expressed antigen 5 antibody
      • MGEA 5 antibody
      • Mgea5 antibody
      • N-acetyl-beta-D-glucosaminidase antibody
      • N-acetyl-beta-glucosaminidase antibody
      • NCOAT antibody
      • Nuclear cytoplasmic O GlcNAcase and acetyltransferase antibody
      • Nuclear cytoplasmic O-GlcNAcase and acetyltransferase antibody
      • OGA antibody
      • OGA_HUMAN antibody
      • Protein O-GlcNAcase antibody
      see all


    • ab124807, at 1/100 dilution, staining MGEA5 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
    • All lanes : Anti-MGEA5 antibody [EPR7154(B)] (ab124807) at 1/10000 dilution

      Lane 1 : HeLa lysate
      Lane 2 : BxPC-3 lysate
      Lane 3 : JAR lysate
      Lane 4 : Fetal brain lysate

      Lysates/proteins at 10 µg per lane.

      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 102 kDa
      Observed band size: 130 kDa (why is the actual band size different from the predicted?)

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD


    This product has been referenced in:
    • Park HC  et al. Urinary N-acetyl-ß-D glucosaminidase as a surrogate marker for renal function in autosomal dominant polycystic kidney disease: 1 year prospective cohort study. BMC Nephrol 13:93 (2012). IHC-Fr ; Human . Read more (PubMed: 22935351) »

    See 1 Publication for this product

    Customer reviews and Q&As

    As discussed in my previous email, I have now arranged for the immunizing peptide to be available from our catalogue. This can be viewed under the catalogue number ab155521:

    I hope this information has be...

    Read More
    Abcam guarantees this product to work in the species/application used in this Abreview.
    Western blot
    Mouse Cell lysate - whole cell (Mouse embryonic stem cell)
    Loading amount
    20 µg
    Mouse embryonic stem cell
    Gel Running Conditions
    Reduced Denaturing (4-12%, MOPS buffer)
    Blocking step
    Milk as blocking agent for 10 minute(s) · Concentration: 5% · Temperature: 20°C

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    Verified customer

    Submitted May 07 2012


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