Overview

Description

  • Nature
    Synthetic

Associated products

Specifications

Our Abpromise guarantee covers the use of ab155521 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    Blocking - Blocking peptide for Anti-MGEA5 antibody [EPR7154(B)] (ab124807)

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

    Constituent: 20% DMSO

General Info

  • Alternative names
    • Beta-hexosaminidase
    • Beta-N-acetylglucosaminidase
    • Beta-N-acetylhexosaminidase
    • Bifunctional protein NCOAT
    • HEXC
    • Hyaluronidase in meningioma
    • KIAA0679
    • MEA5
    • Meningioma expressed antigen 5
    • Meningioma-expressed antigen 5
    • MGEA 5
    • Mgea5
    • N-acetyl-beta-D-glucosaminidase
    • N-acetyl-beta-glucosaminidase
    • NCOAT
    • Nuclear cytoplasmic O GlcNAcase and acetyltransferase
    • Nuclear cytoplasmic O-GlcNAcase and acetyltransferase
    • OGA
    • OGA_HUMAN
    • Protein O-GlcNAcase
    see all
  • Function
    Isoform 1: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc and 4-methylumbelliferone-GlcNAc as substrates but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro) (PubMed:11148210). Does not bind acetyl-CoA and does not have histone acetyltransferase activity (PubMed:24088714).
    Isoform 3: Cleaves GlcNAc but not GalNAc from O-glycosylated proteins. Can use p-nitrophenyl-beta-GlcNAc as substrate but not p-nitrophenyl-beta-GalNAc or p-nitrophenyl-alpha-GlcNAc (in vitro), but has about six times lower specific activity than isoform 1.
  • Tissue specificity
    Ubiquitous. Shows highest expression in the brain, placenta and pancreas.
  • Sequence similarities
    Belongs to the glycosyl hydrolase 84 family.
  • Post-translational
    modifications
    Proteolytically cleaved by caspase-3 during apoptosis. The fragments interact with each other; cleavage does not decrease enzyme activity.
  • Cellular localization
    Cytoplasm and Nucleus.
  • Information by UniProt

References

ab155521 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for contacting us.
This product is a blocking peptide for antibody ab124807, please do not confuse it with protein. This peptide is biologically inactive which means it cannot act as enzyme.
I hope this information is helpful to you....

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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