Overview

  • Product nameAnti-MGMT antibody
    See all MGMT primary antibodies
  • Description
    Rabbit polyclonal to MGMT
  • Tested applicationsSuitable for: WB, ICCmore details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide corresponding to an internal sequence of Human MGMT.

Properties

Applications

Our Abpromise guarantee covers the use of ab112504 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 22 kDa.
ICC Use a concentration of 1 µg/ml.

Target

  • FunctionInvolved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.
  • Sequence similaritiesBelongs to the MGMT family.
  • Cellular localizationNucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • 6 O methylguanine DNA methyltransferase antibody
    • 6-O-methylguanine-DNA methyltransferase antibody
    • Agat antibody
    • AGT antibody
    • AI267024 antibody
    • EC 2.1.1.63 antibody
    • Methylated DNA protein cysteine methyltransferase antibody
    • Methylated-DNA--protein-cysteine methyltransferase antibody
    • Methylguanine DNA methyltransferase antibody
    • MGC107020 antibody
    • MGMT antibody
    • MGMT_HUMAN antibody
    • O 6 methylguanine DNA alkyltransferase antibody
    • O 6 methylguanine DNA methyltransferase antibody
    • O-6-methylguanine-DNA methyltransferase antibody
    • O-6-methylguanine-DNA-alkyltransferase antibody
    see all

Anti-MGMT antibody images

  • All lanes : Anti-MGMT antibody (ab112504)

    Lane 1 : Rat brain tissue lysate
    Lane 2 : Rat liver tissue lysate
    Lane 3 : Rat spleen tissue lysate
    Lane 4 : Rat thymus tissue lysate
    Lane 5 : MCF-7 whole cell lysate
    Lane 6 : HeLa whole cell lysate
    Lane 7 : Jurkat whole cell lysate
    Lane 8 : Raji whole cell lysate


    Predicted band size : 22 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • ab112504 staining MGMT in Lung cancer by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

References for Anti-MGMT antibody (ab112504)

ab112504 has not yet been referenced specifically in any publications.

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