Anti-MGMT antibody (ab69629)
Key features and details
- Rabbit polyclonal to MGMT
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
-
Product name
Anti-MGMT antibody
See all MGMT primary antibodies -
Description
Rabbit polyclonal to MGMT -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide derived from the internal region of human MGMT.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS -
Concentration information loading...
-
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).
|
Notes |
---|
WB
1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
-
Function
Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. -
Sequence similarities
Belongs to the MGMT family. -
Cellular localization
Nucleus. - Information by UniProt
-
Database links
- Entrez Gene: 4255 Human
- Entrez Gene: 25332 Rat
- Omim: 156569 Human
- SwissProt: P16455 Human
- SwissProt: P24528 Rat
- Unigene: 501522 Human
- Unigene: 9836 Rat
-
Alternative names
- 6 O methylguanine DNA methyltransferase antibody
- 6-O-methylguanine-DNA methyltransferase antibody
- Agat antibody
see all
Images
Datasheets and documents
-
SDS download
-
Datasheet download
References (6)
ab69629 has been referenced in 6 publications.
- Hu YH et al. Regulation of temozolomide resistance in glioma cells via the RIP2/NF-?B/MGMT pathway. CNS Neurosci Ther 27:552-563 (2021). PubMed: 33460245
- Lv W et al. Differentiated embryonic chondrocyte-expressed gene 1 promotes temozolomide resistance by modulating the SP1-MGMT axis in glioblastoma. Am J Transl Res 13:2331-2349 (2021). PubMed: 34017393
- Yang HC et al. Resveratrol restores sensitivity of glioma cells to temozolamide through inhibiting the activation of Wnt signaling pathway. J Cell Physiol 234:6783-6800 (2019). PubMed: 30317578
- Moen EL et al. Characterization of CpG sites that escape methylation on the inactive human X-chromosome. Epigenetics 10:810-8 (2015). PubMed: 26178744
- Ishiguro K et al. Expression of O(6)-Methylguanine-DNA Methyltransferase Examined by Alkyl-Transfer Assays, Methylation-Specific PCR and Western Blots in Tumors and Matched Normal Tissue. J Cancer Ther 4:919-931 (2013). PubMed: 23946891
- Vlashi E et al. Differential Effects of the Proteasome Inhibitor NPI-0052 against Glioma Cells. Transl Oncol 3:50-5 (2010). WB ; Human . PubMed: 20165695