MGMT overexpression 293T lysate (whole cell) (ab94051)

Overview

  • Product nameMGMT overexpression 293T lysate (whole cell)
  • Description
    MGMT overexpression lysate
  • General notesab94051 is a 293T cell transfected lysate in which Human MGMT has been transiently over-expressed using a pCMV-MGMT plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
  • Tested applicationsSuitable for: WBmore details

Properties

  • BackgroundFunction: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated. Similarity: Belongs to the MGMT family.

Applications

Our Abpromise guarantee covers the use of ab94051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution.

MGMT overexpression 293T lysate (whole cell) images

  • ab94051 at 15µg/lane on an SDS-PAGE gel
  • All lanes : Anti-MGMT antibody (ab55442) at 1/500 dilution

    Lane 1 : MGMT overexpression 293T lysate (whole cell) (ab94051)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

References for MGMT overexpression 293T lysate (whole cell) (ab94051)

ab94051 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"