Anti-MGP antibody (ab86233)

Overview

  • Product nameAnti-MGP antibody
    See all MGP primary antibodies
  • Description
    Rabbit polyclonal to MGP
  • Tested applicationsSuitable for: IHC-P, WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rat, Guinea pig
  • Immunogen

    Synthetic peptide corresponding to a region within the internal sequence amino acids 36-85 of Human MGP (

    NRRNANTFISPQQRWRAKVQERIRERSKPVHELNREACDDYRLCERYAMV

    ) NP_000891

  • Positive control
    • Jurkat cell lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab86233 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 2.5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

Target

  • FunctionAssociates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
  • Involvement in diseaseDefects in MGP are the cause of Keutel syndrome (KS) [MIM:245150]. KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.
  • Sequence similaritiesBelongs to the osteocalcin/matrix Gla protein family.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
  • Post-translational
    modifications
    Requires vitamin K-dependent gamma-carboxylation for its function.
  • Cellular localizationSecreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Cell growth inhibiting gene 36 protein antibody
    • Cell growth-inhibiting gene 36 protein antibody
    • GAMMA-CARBOXYGLUTAMIC ACID PROTEIN, MATRIX antibody
    • GIG36 antibody
    • Matrix Gla protein antibody
    • MGLAP antibody
    • MGP antibody
    • MGP_HUMAN antibody
    • NTI antibody
    see all

Anti-MGP antibody images

  • Anti-MGP antibody (ab86233) at 1 µg/ml + Jurkat cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 12 kDa
    Observed band size : 12 kDa
    Additional bands at : 30 kDa,>60 kDa. We are unsure as to the identity of these extra bands.
    Gel concentration: 10-20%
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human small intestine tissue labelling MGP with ab86233 at 2.5µg/ml.

References for Anti-MGP antibody (ab86233)

ab86233 has not yet been referenced specifically in any publications.

Product Wall

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Aorta)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: NaCitrate 10mM
Permeabilization No
Specification Aorta
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative Formaldehyde
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Submitted Aug 03 2015

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The anti-MGP antibody (ab70193) has been raised against the full length MGP protein and as it is a polyclonal antibody the exact epitope cannot be determined.We would expect theantibodyto be able to detectboth for...

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