The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 2.5 µg/ml.
Use a concentration of 1 µg/ml. Predicted molecular weight: 12 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
Involvement in disease
Defects in MGP are the cause of Keutel syndrome (KS) [MIM:245150]. KS is an autosomal recessive disorder characterized by abnormal cartilage calcification, peripheral pulmonary stenosis neural hearing loss and midfacial hypoplasia.
Belongs to the osteocalcin/matrix Gla protein family. Contains 1 Gla (gamma-carboxy-glutamate) domain.
Requires vitamin K-dependent gamma-carboxylation for its function.
Suna G et al. Extracellular Matrix Proteomics Reveals Interplay of Aggrecan and Aggrecanases in Vascular Remodeling of Stented Coronary Arteries. Circulation137:166-183 (2018).
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