Overview

  • Product nameAnti-MGST3 antibody [2G10CG7]
    See all MGST3 primary antibodies
  • Description
    Mouse monoclonal [2G10CG7] to MGST3
  • Tested applicationsSuitable for: ICC/IF, In-Cell ELISA, IPmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    purified enzymes from bovine liver

  • Positive control
    • ICC/IF: HDFn cells IP: Human and Rat liver lysates
  • General notes

    Product was previously marketed under the MitoSciences sub-brand.

Properties

Applications

Our Abpromise guarantee covers the use of ab110309 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 2 µg/ml.
In-Cell ELISA Use a concentration of 2 µg/ml. (0.2 µg/well)
IP Use at an assay dependent dilution.

Target

  • FunctionAlso functions as a glutathione peroxidase.
  • Tissue specificityPredominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes.
  • Sequence similaritiesBelongs to the MAPEG family.
  • Cellular localizationEndoplasmic reticulum membrane. Microsome membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mgst3 antibody
    • MGST3_HUMAN antibody
    • Microsomal glutathione S transferase 3 antibody
    • Microsomal glutathione S-transferase 3 antibody
    • Microsomal GST-3 antibody
    • Microsomal GST-III antibody
    • Microsomal GST3 antibody
    • Microsomal GSTIII antibody
    see all

Anti-MGST3 antibody [2G10CG7] images

  • Immunocytochemistry image of ab110309-stained human HDFn cells. The cells were paraformaldehyde fixed (4%, 20 minutes) and Triton X-100 permeabilized (0.1%, 15 minutes). The cells were incubated with ab110309 at 2 µg/ml for 2 hours at room temperature or over night at 4°C. The secondary antibody was (green) Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour. 10% Goat serum was used as the blocking agent for all blocking steps. Target protein locates primarily in the mitochondria.
  • Using MitoSciences' standard immunoprecipitation protocol, ab110309 pulls down the 15kDa MGST3 protein from human and rat liver samples. HepG2 lysate and mouse liver lysate yielded smaller amounts. This identity of this protein was confirmed by mass spectrometry. This gel was stained with coomassie.

References for Anti-MGST3 antibody [2G10CG7] (ab110309)

ab110309 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"