Recombinant
RabMAb

Anti-MGST3 antibody [EPR12352] (ab192254)

Overview

  • Product name
    Anti-MGST3 antibody [EPR12352]
    See all MGST3 primary antibodies
  • Description
    Rabbit monoclonal [EPR12352] to MGST3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-P, IP, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human MGST3 aa 1-100. The exact sequence is proprietary.
    Database link: O14880

  • Positive control
    • A673, HepG2, Human fetal brain, and mouse spleen lysates; Human and mouse cardiac muscle and rat liver tissues.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab192254 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/20.
WB 1/1000 - 1/10000. Detects a band of approximately 17 kDa (predicted molecular weight: 17 kDa).

Target

  • Function
    Also functions as a glutathione peroxidase.
  • Tissue specificity
    Predominantly expressed in heart, skeletal muscle, and adrenal cortex. Also found in brain, placenta, liver, kidney, pancreas, thyroid, testis and ovary. Almost absent in lung, thymus and peripheral blood leukocytes.
  • Sequence similarities
    Belongs to the MAPEG family.
  • Cellular localization
    Endoplasmic reticulum membrane. Microsome membrane. Microsome membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • Mgst3 antibody
    • MGST3_HUMAN antibody
    • Microsomal glutathione S transferase 3 antibody
    • Microsomal glutathione S-transferase 3 antibody
    • Microsomal GST-3 antibody
    • Microsomal GST-III antibody
    • Microsomal GST3 antibody
    • Microsomal GSTIII antibody
    see all

Images

  • All lanes : Anti-MGST3 antibody [EPR12352] (ab192254) at 1/10000 dilution

    Lane 1 : A673 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of paraffin-embedded Human cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control).

  • Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 17 kDa

  • Anti-MGST3 antibody [EPR12352] (ab192254) at 1/1000 dilution + mouse spleen lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 17 kDa

  • Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control).

  • Immunohistochemical analysis of paraffin-embedded rat liver tissue labeling MGST3 with ab192254 at 1/500 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin.
    (inset: negative control).

  • Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab192254 at 1/20 dilution.
    Secondary: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution.

References

ab192254 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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