Anti-MHC Class 1 H2 Db antibody [27-11-13] (ab25244)

Overview

  • Product name
    Anti-MHC Class 1 H2 Db antibody [27-11-13]
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db
  • Specificity
    ab25244 recognizes the a3 domain of H2Db class I MHC antigen. The antibody cross-reacts with the a3 domain of H2Ld, H2Dq and H2Lq, but not H2Kd or H2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • Tested applications
    Suitable for: Functional Studies, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C3H.SW splenocytes (Mouse)

  • Epitope
    Recognizes an epitope on the a chain of H2Db.
  • General notes


    ab25244 can be used for complement-dependent cytoxicity assays.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 8.20
    Constituent: 100% Borate buffered saline
  • Concentration information loading...
  • Primary antibody notes
    ab25244 can be used for complement-dependent cytoxicity assays.
  • Clonality
    Monoclonal
  • Clone number
    27-11-13
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25244 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Complement-dependent cytoxicity assay.

Flow Cyt Use at an assay dependent concentration. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localization
    Cell Membrane; single pass type I membrane protein
  • Database links
  • Alternative names
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

References

This product has been referenced in:
  • Dower SK  et al. The interaction of monoclonal antibodies with MHC class I antigens on mouse spleen cells. I. Analysis of the mechanism of binding. J Immunol 132:751-8 (1984). Read more (PubMed: 6690617) »

See 1 Publication for this product

Customer reviews and Q&As

Merci pour votre réponse. Je suis désolée que le ab25334 ne convient donc pas. Je vous transmet les détails du code pour tester le ab25244. Veuillez noter que la remise de 100% est applicable sur un anticorps ultérieur, après que nous avons reçu le fee...

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Merci pour votre émail.
Je m'excuse du délais de ma réponse. Effectivement, en discutant avec une collègue, j'ai trouvé que nous avons aussi le clone 34-1-2 dans notre catalogue. Dans la publication il me semblait donner une meilleure valeur pour ...

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Merci de votre intérêt pour ab25244.

J'ai contacté le laboratoire pour avoir plus de détails sur les études fonctionnelles faites avec cet anticorps. Dans l'article ci-dessus les auteurs ont utilisé ce clone pour des études d'inhibition. Read More

Thank you very much for your valuable feedback. We are glad to hear the antibody is working well with your new protocol. We would be very grateful if you could submit an Abreview about this product via the online product datasheet. We always encoura...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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