Anti-MHC Class 1 H2 Db antibody [27-11-13] (Biotin) (ab25231)

Overview

  • Product name
    Anti-MHC Class 1 H2 Db antibody [27-11-13] (Biotin)
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db (Biotin)
  • Conjugation
    Biotin
  • Specificity
    This antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) - C3H.SW mouse splenocytes.

  • Epitope
    This antibody reacts with an epitope on the alpha chain of H2Db.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    27-11-13
  • Isotype
    IgG2a
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
IHC-Fr
  • Application notes
    Flow Cyt: Use 1µg for 106 cells.
    IHC-Fr[acetone fixed]: Use at an assay dependent dilution.
    Complement-dependent cytoxicity assay: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
    • Cellular localization
      Cell Membrane; single pass type I membrane protein
    • Database links
    • Alternative names
      • H 2D cell surface glycoprotein antibody
      • H2 class I histocompatibility antigen, DB alpha chain antibody
      • H2D(B) antibody
      • H2D1 antibody
      • Histocompatibility 2, D region locus 1 antibody
      see all

    References

    ab25231 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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