Anti-MHC Class 1 H2 Db antibody [27-11-13] (FITC) (ab25028)

Overview

  • Product nameAnti-MHC Class 1 H2 Db antibody [27-11-13] (FITC)
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab25028 recognizes with the a3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the a3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • Tested applicationsSuitable for: Flow Cyt, IHC-Fr, Functional Studiesmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse C3H.SW splenocytes

  • EpitopeAn epitope on the a chain of H-2Db

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number27-11-13
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration. (Complement-dependent cytoxicity assay)

Target

  • RelevanceIn the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localizationCell Membrane; single pass type I membrane protein
  • Database links
  • Alternative names
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

References for Anti-MHC Class 1 H2 Db antibody [27-11-13] (FITC) (ab25028)

ab25028 has not yet been referenced specifically in any publications.

Product Wall

Both ab21321 and ab25028 recognize extracellular epitopes and can be used to stain live cells for FACS.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"