Tissue/ cell preparation (Mouse) -C3H.SW splenocytes.
Our Abpromise guarantee covers the use of ab25547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent dilution. (Complement-dependent cytoxicity assay)|
|Flow Cyt||Use 0.2µg for 106 cells.|
|IHC-Fr||Use at an assay dependent dilution. Acetone fixed only.|