Anti-MHC Class 1 H2 Db antibody [27-11-13] (Phycoerythrin) (ab25547)

Overview

  • Product nameAnti-MHC Class 1 H2 Db antibody [27-11-13] (Phycoerythrin)
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • Specificityab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed.
  • Tested applicationsSuitable for: Functional Studies, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse) -C3H.SW splenocytes.

  • EpitopeThis antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 16% Sucrose, PBS and stabilizing agent.
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number27-11-13
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25547 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent dilution. (Complement-dependent cytoxicity assay)
Flow Cyt Use 0.2µg for 106 cells.
IHC-Fr Use at an assay dependent dilution. Acetone fixed only.

Target

  • RelevanceIn the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localizationCell Membrane; single pass type I membrane protein
  • Database links
  • Alternative names
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

Anti-MHC Class 1 H2 Db antibody [27-11-13] (Phycoerythrin) images

References for Anti-MHC Class 1 H2 Db antibody [27-11-13] (Phycoerythrin) (ab25547)

This product has been referenced in:
  • Hoerter JA  et al. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC. J Exp Med 210:1807-21 (2013). Read more (PubMed: 23940257) »

See 1 Publication for this product

Product Wall

Thank you for your enquiry. We do not have a protocol specifically for acetone fixation. However, may I recommend the excellent resource at IHC world. The following link details technical tips to consider when performing acetone fixation. http:...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"