PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13] (ab25547)
Key features and details
- PE Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: PE. Ex: 488nm, Em: 575nm
- Isotype: IgG2a
Related conjugates and formulations
Overview
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Product name
PE Anti-MHC Class 1 H2 Db/H2-D1 antibody [27-11-13]
See all MHC Class 1 H2 Db/H2-D1 primary antibodies -
Description
PE Mouse monoclonal [27-11-13] to MHC Class 1 H2 Db/H2-D1 -
Host species
Mouse -
Conjugation
PE. Ex: 488nm, Em: 575nm -
Specificity
ab25547 recognizes the alpha3 domain of H-2Db class I MHC antigen. The antibody cross-reacts with the alpha3 domain of H-2Ld, H-2Dq and H-2Lq, but not H-2Kd or H-2Dd. Reactivity with haplotypes k, f, p, r, and s has not been observed. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse MHC Class 1 H2 Db/H2-D1. BDF1 mouse splenocytes
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Epitope
This antibody reacts with an epitope on the alpha chain [alpha3 domain] of H2Db. -
Positive control
- Flow cyt: Mouse BALB/c splenocytes.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Store In the Dark. -
Storage buffer
pH: 7.3
Preservative: 0.09% Sodium azide
Constituents: PBS, 16% Sucrose
Also contains a stabilizing agent. -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
27-11-13 -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab25547 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 0.2µg for 106 cells.
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Notes |
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Flow Cyt
Use 0.2µg for 106 cells. |
Target
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Relevance
In the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells. -
Cellular localization
Cell Membrane; single pass type I membrane protein -
Database links
- Entrez Gene: 14964 Mouse
- SwissProt: P01899 Mouse
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Alternative names
- H 2D cell surface glycoprotein antibody
- H2 class I histocompatibility antigen, DB alpha chain antibody
- H2D(B) antibody
see all
Images
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab25547 has been referenced in 1 publication.
- Hoerter JA et al. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC. J Exp Med 210:1807-21 (2013). PubMed: 23940257