Anti-MHC Class 1 H2 Db antibody [28-14-8] (FITC) (ab25056)


  • Product nameAnti-MHC Class 1 H2 Db antibody [28-14-8] (FITC)
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [28-14-8] to MHC Class 1 H2 Db (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityAb25056 binds to the a3 domain of H-2Db in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. Ab25056 has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse)-C3H.SW mouse splenocytes.

  • EpitopeThis antibody recognises an epitope in the a3 domain of H-2Db.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number28-14-8
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab25056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


  • RelevanceIn the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localizationCell Membrane; single pass type I membrane protein
  • Database links
  • Alternative names
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

Anti-MHC Class 1 H2 Db antibody [28-14-8] (FITC) images

  • ab25056 (at a concentration of 1 µg / 106 cells) staining splenocytes from DBA1/J mice by flow cytometry. Small lymphocytes were gated and analyzed on a FACScanTM flow cytometer (BDIS, San Jose, CA).

References for Anti-MHC Class 1 H2 Db antibody [28-14-8] (FITC) (ab25056)

This product has been referenced in:
  • Hoerter JA  et al. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC. J Exp Med 210:1807-21 (2013). Read more (PubMed: 23940257) »

See 1 Publication for this product

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