Anti-MHC Class 1 H2 Db antibody [28-14-8] (FITC) (ab25056)

Overview

  • Product nameAnti-MHC Class 1 H2 Db antibody [28-14-8] (FITC)
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [28-14-8] to MHC Class 1 H2 Db (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityAb25056 binds to the a3 domain of H-2Db in the presence or absence of ß2 microglobulin. It cross reacts with the a3 domain of H-2Ld, but not Kd or Dd, and with H-2Dq and/or Lq. Ab25056 has been shown to block H-2Ld-specific and H-2Ld-restricted antigen-specific lysis of target cells by cytotoxic T lymphocytes, but it does not block recognition of H-2Ld-positive target cells by Ly-6G2-positive NK cells.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Tissue/ cell preparation (Mouse)-C3H.SW mouse splenocytes.

  • EpitopeThis antibody recognises an epitope in the a3 domain of H-2Db.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityMonoclonal
  • Clone number28-14-8
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab25056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceIn the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localizationCell Membrane; single pass type I membrane protein
  • Database links
  • Alternative names
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

Anti-MHC Class 1 H2 Db antibody [28-14-8] (FITC) images

  • ab25056 (at a concentration of 1 µg / 106 cells) staining splenocytes from DBA1/J mice by flow cytometry. Small lymphocytes were gated and analyzed on a FACScanTM flow cytometer (BDIS, San Jose, CA).

References for Anti-MHC Class 1 H2 Db antibody [28-14-8] (FITC) (ab25056)

This product has been referenced in:
  • Hoerter JA  et al. Coreceptor affinity for MHC defines peptide specificity requirements for TCR interaction with coagonist peptide-MHC. J Exp Med 210:1807-21 (2013). Read more (PubMed: 23940257) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab25056.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"