Anti-MHC Class 1 H2 Db antibody [B22-249.R1] (FITC) (ab114065)

Overview

  • Product nameAnti-MHC Class 1 H2 Db antibody [B22-249.R1] (FITC)
    See all MHC Class 1 H2 Db primary antibodies
  • Description
    Mouse monoclonal [B22-249.R1] to MHC Class 1 H2 Db (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Mouse C57BL/6 lymphocytes.

  • Positive control
    • Mouse Spleen cells (C57BL/6 strain).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituents: 99% PBS, 0.5% BSA
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberB22-249.R1
  • MyelomaNS1
  • IsotypeIgG2a
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab114065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 1µg for 106 cells. ab18455-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceIn the mouse the MHC Class 1 loci are called H2K, H2L and H2D. The equivalent loci in the human MHC are HLA (Human Leukocyte Antigen). MHC class I molecules are heterodimers, consisting of a single transmembrane polypeptide chain (the a-chain) which is highly polymorphic, and the invariant ß2 microglobulin (which is encoded elsewhere, not in the MHC). MHC class I molecules are found on almost every nucleated cell of the body. Their major function is to present peptide fragments derived from antigens to cytotoxic T cells.
  • Cellular localizationCell Membrane; single pass type I membrane protein
  • Database links
  • Alternative names
    • H 2D cell surface glycoprotein antibody
    • H2 class I histocompatibility antigen, DB alpha chain antibody
    • H2D(B) antibody
    • H2D1 antibody
    • Histocompatibility 2, D region locus 1 antibody
    see all

Anti-MHC Class 1 H2 Db antibody [B22-249.R1] (FITC) images

  • ab114065 at 1µg/106 cells staining MHC Class 1 H2 Db in Mouse spleen cells (C57BL/6 strain) by Flow Cytometry.
    Percentage of cells stained above control (FITC Mouse IgG2a): 99.11%.

References for Anti-MHC Class 1 H2 Db antibody [B22-249.R1] (FITC) (ab114065)

ab114065 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab114065.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"