FITC Anti-MHC class I antibody [34-1-2S] (ab95572)
Key features and details
- FITC Mouse monoclonal [34-1-2S] to MHC class I
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-MHC class I antibody [34-1-2S]
See all MHC class I primary antibodies -
Description
FITC Mouse monoclonal [34-1-2S] to MHC class I -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Specificity
ab95572 reacts with the mouse MHC class I, H-2Kd and H-2Dd. This cytotoxic antibody also cross reacts with K b,s,r,q,p -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Tissue, cells or virus corresponding to Mouse MHC class I. C3H.SW Mouse splenocytes
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Positive control
- Mouse splenocytes
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.20
Preservative: 0.09% Sodium azide
Constituents: 0.87% Sodium chloride, PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
34-1-2S -
Isotype
IgG2a -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab95572 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
Use 1µg for 105-8 cells.
Staining the cell sample in a final volume of 100 µL is recommended.
ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
Use 1µg for 105-8 cells. Staining the cell sample in a final volume of 100 µL is recommended.
ab18455 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
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Target
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Relevance
MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen. -
Cellular localization
Cell Membrane; Type I membrane protein. -
Alternative names
- A 28 antibody
- A 9 antibody
- Antigen presenting molecule antibody
see all
Images
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab95572 has been referenced in 3 publications.
- Wu X et al. WEE1 inhibitor and ataxia telangiectasia and RAD3-related inhibitor trigger stimulator of interferon gene-dependent immune response and enhance tumor treatment efficacy through programmed death-ligand 1 blockade. Cancer Sci 112:4444-4456 (2021). PubMed: 34382294
- Bent EH et al. Microenvironmental IL-6 inhibits anti-cancer immune responses generated by cytotoxic chemotherapy. Nat Commun 12:6218 (2021). PubMed: 34711820
- Okazaki K et al. Enhancer remodeling promotes tumor-initiating activity in NRF2-activated non-small cell lung cancers. Nat Commun 11:5911 (2020). PubMed: 33219226