Anti-MHC class I antibody [AF6-88.5.5.3] (Biotin) (ab93528)

Overview

  • Product name
    Anti-MHC class I antibody [AF6-88.5.5.3] (Biotin)
    See all MHC class I primary antibodies
  • Description
    Mouse monoclonal [AF6-88.5.5.3] to MHC class I (Biotin)
  • Conjugation
    Biotin
  • Specificity
    Reacts with the H-2Kb MHC class I alloantigen. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    C57BL Mouse splenocytes.

  • Positive control
    • Splenocytes

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: aqueous buffer
  • Concentration information loading...
  • Purity
    IgG fraction
  • Clonality
    Monoclonal
  • Clone number
    AF6-88.5.5.3
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab93528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 0.06µg for 105-8 cells in a final volume of 100µL.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
    • Cellular localization
      Cell Membrane; Type I membrane protein.
    • Alternative names
      • A 28 antibody
      • A 9 antibody
      • Antigen presenting molecule antibody
      • Aw 24 antibody
      • Aw 68 antibody
      • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
      • H2 K1 antibody
      • H2K antibody
      • HLA A antibody
      • HLA class I histocompatibility antigen A 1 alpha chain antibody
      • HLA class I histocompatibility antigen A 11 alpha chain antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A 24 alpha chain antibody
      • HLA class I histocompatibility antigen A 3 alpha chain antibody
      • HLA class I histocompatibility antigen A 30 alpha chain antibody
      • HLA class I histocompatibility antigen A 32 alpha chain antibody
      • HLA class I histocompatibility antigen A 68 alpha chain antibody
      • HLAA antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • MHC class I antigen A 1 antibody
      • MHC class I antigen A*11 antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A*24 antibody
      • MHC class I antigen A*3 antibody
      • MHC class I antigen A*30 antibody
      • MHC class I antigen A*32 antibody
      • MHC class I antigen A*68 antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC class I heavy chain H2 K antibody
      see all

    Images

    • Staining of C57Bl/6 splenocytes with 0.06µg of biotin Mouse IgG2a, K Isotype control (left) or 0.06 µg of ab93528(right), followed by Streptavidin-PE.
      Total viable cells were used for analysis.

    References

    ab93528 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab93528.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

    Sign up