Overview

  • Product nameAnti-MHC class I antibody [CVS 22]
    See all MHC class I primary antibodies
  • Description
    Mouse monoclonal [CVS 22] to MHC class I
  • Specificityab23491 reacts with EQUINE MHC CLASS I
  • Tested applicationsSuitable for: IHC-Fr, IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Horse
  • Immunogen

    Tissue/ cell preparation (Equine leucocytes).

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 5 - 10% Foetal calf serum, Tissue culture supernatant, 0.4M Tris HCl. pH 7.4
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberCVS 22
  • Myelomax63-Ag8.653
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23491 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
Flow Cyt Use 10µl for 106 cells. ab170191-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • Cellular localizationCell Membrane; Type I membrane protein.
  • Alternative names
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

References for Anti-MHC class I antibody [CVS 22] (ab23491)

This product has been referenced in:
  • Quinn BA  et al. Changes in major proteins in the embryonic capsule during immobilization (fixation) of the conceptus in the third week of pregnancy in the mare. Reproduction 134:161-70 (2007). Read more (PubMed: 17641098) »

See 1 Publication for this product

Product Wall

We have not added WB as an application as the reference does not provide direct evidence of suitability. The article just says that ß2M is not associated MHC class 1 heavy chain in their samples, so it could be that the WB results were negative and th...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"