Anti-MHC class I antibody [ER-HR 52] (ab15681)

Overview

  • Product name
    Anti-MHC class I antibody [ER-HR 52]
    See all MHC class I primary antibodies
  • Description
    Rat monoclonal [ER-HR 52] to MHC class I
  • Specificity
    Ab15681 detects MHC class I antigens of d and b haplotypes.
  • Tested applications
    Suitable for: IHC-Fr, IHC (PFA fixed)more details
    Unsuitable for: IHC-FoFr
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Murine macrophage precursor cells.

  • General notes


    Ab15681 detects MHC class I antigens and is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA. pH 7.2
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Primary antibody notes
    Ab15681 detects MHC class I antigens and is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.
  • Clonality
    Monoclonal
  • Clone number
    ER-HR 52
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml. The determinant recognised by ab15681 is glutaraldehyde (0.05%), paraformaldehyde (1%) and acetone-resistant.
IHC (PFA fixed) 1/300. PubMed: 17446933
  • Application notes
    Is unsuitable for IHC-FoFr.
  • Target

    • Relevance
      MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
    • Cellular localization
      Cell Membrane; Type I membrane protein.
    • Alternative names
      • A 28 antibody
      • A 9 antibody
      • Antigen presenting molecule antibody
      • Aw 24 antibody
      • Aw 68 antibody
      • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
      • H2 K1 antibody
      • H2K antibody
      • HLA A antibody
      • HLA class I histocompatibility antigen A 1 alpha chain antibody
      • HLA class I histocompatibility antigen A 11 alpha chain antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A 24 alpha chain antibody
      • HLA class I histocompatibility antigen A 3 alpha chain antibody
      • HLA class I histocompatibility antigen A 30 alpha chain antibody
      • HLA class I histocompatibility antigen A 32 alpha chain antibody
      • HLA class I histocompatibility antigen A 68 alpha chain antibody
      • HLAA antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • MHC class I antigen A 1 antibody
      • MHC class I antigen A*11 antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A*24 antibody
      • MHC class I antigen A*3 antibody
      • MHC class I antigen A*30 antibody
      • MHC class I antigen A*32 antibody
      • MHC class I antigen A*68 antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC class I heavy chain H2 K antibody
      see all

    Images

    • Immunohistochemical analysis of frozen mouse brain tissue, post-infection with Salmonella typhimurium, staining MHC Class I with ab15681.
      Tissue was blocked with 2% BSA, 10% normal rabbit serum, before incubating with primary antibody overnight. A biotinylated anti-rat IgG was used as the secondary antibody. Staining was detected using DAB.

    References

    This product has been referenced in:
    • Saranchova I  et al. Discovery of a Metastatic Immune Escape Mechanism Initiated by the Loss of Expression of the Tumour Biomarker Interleukin-33. Sci Rep 6:30555 (2016). IHC-Fr . Read more (PubMed: 27619158) »
    • Placke T  et al. Platelet-derived MHC class I confers a pseudonormal phenotype to cancer cells that subverts the antitumor reactivity of natural killer immune cells. Cancer Res 72:440-8 (2012). ICC/IF . Read more (PubMed: 22127925) »

    See all 7 Publications for this product

    Customer reviews and Q&As

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (tumor tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrate buffer
    Permeabilization
    Yes - 0.3% triton X-100
    Specification
    tumor tissue
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Fixative
    Paraformaldehyde
    Username

    Abcam user community

    Verified customer

    Submitted Jun 14 2017

    Can you please tell me what kind of tissue you are trying to stain (species and organ), how long it was fixed in formalin, and if you included an antigen retrievals step before incubation with the antibody?

    The application that is listed on ...

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    My apologies for the late reply, I was away last week. I have received the following information from the source of ab15681: 1. Blocking endogenous biotin is important with the detection system used (biotinylated secondary). This may be improved. ...

    Read More

    I'm very sorry to hear you are having a problem with ab15681. This antibody has not been tested in IHC on perfusion formaldehyde fixed sections, we recommend using fresh frozen sections followed by postfixation in glutaraldehyde (0.05%), paraformaldehy...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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