Overview

  • Product nameAnti-MHC class I antibody [ER-HR 52]
    See all MHC class I primary antibodies
  • Description
    Rat monoclonal [ER-HR 52] to MHC class I
  • SpecificityAb15681 detects MHC class I antigens of d and b haplotypes.
  • Tested applicationsSuitable for: IHC-Fr, IHC (PFA fixed)more details
    Unsuitable for: IHC-FoFr
  • Species reactivity
    Reacts with: Mouse
  • Immunogen

    Murine macrophage precursor cells.

  • General notes


    Ab15681 detects MHC class I antigens and is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: PBS, 10mg/ml BSA. pH 7.2
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Primary antibody notesAb15681 detects MHC class I antigens and is therefore a valuable tool for studying cytotoxic T-cell interactions with class I positive antigen presenting cells.
  • ClonalityMonoclonal
  • Clone numberER-HR 52
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15681 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use a concentration of 1 µg/ml. The determinant recognised by ab15681 is glutaraldehyde (0.05%), paraformaldehyde (1%) and acetone-resistant.
IHC (PFA fixed) 1/300. PubMed: 17446933
  • Application notesIs unsuitable for IHC-FoFr.
  • Target

    • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
    • Cellular localizationCell Membrane; Type I membrane protein.
    • Alternative names
      • A 28 antibody
      • A 9 antibody
      • Antigen presenting molecule antibody
      • Aw 24 antibody
      • Aw 68 antibody
      • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
      • H2 K1 antibody
      • H2K antibody
      • HLA A antibody
      • HLA class I histocompatibility antigen A 1 alpha chain antibody
      • HLA class I histocompatibility antigen A 11 alpha chain antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A 24 alpha chain antibody
      • HLA class I histocompatibility antigen A 3 alpha chain antibody
      • HLA class I histocompatibility antigen A 30 alpha chain antibody
      • HLA class I histocompatibility antigen A 32 alpha chain antibody
      • HLA class I histocompatibility antigen A 68 alpha chain antibody
      • HLAA antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • MHC class I antigen A 1 antibody
      • MHC class I antigen A*11 antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A*24 antibody
      • MHC class I antigen A*3 antibody
      • MHC class I antigen A*30 antibody
      • MHC class I antigen A*32 antibody
      • MHC class I antigen A*68 antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC class I heavy chain H2 K antibody
      see all

    Anti-MHC class I antibody [ER-HR 52] images

    • Immunohistochemical analysis of frozen mouse brain tissue, post-infection with Salmonella typhimurium, staining MHC Class I with ab15681.
      Tissue was blocked with 2% BSA, 10% normal rabbit serum, before incubating with primary antibody overnight. A biotinylated anti-rat IgG was used as the secondary antibody. Staining was detected using DAB.

    References for Anti-MHC class I antibody [ER-HR 52] (ab15681)

    This product has been referenced in:
    • Placke T  et al. Platelet-derived MHC class I confers a pseudonormal phenotype to cancer cells that subverts the antitumor reactivity of natural killer immune cells. Cancer Res 72:440-8 (2012). ICC/IF . Read more (PubMed: 22127925) »
    • Püntener U  et al. Long-term impact of systemic bacterial infection on the cerebral vasculature and microglia. J Neuroinflammation 9:146 (2012). IHC-Fr ; Mouse . Read more (PubMed: 22738332) »

    See all 6 Publications for this product

    Product Wall

    Can you please tell me what kind of tissue you are trying to stain (species and organ), how long it was fixed in formalin, and if you included an antigen retrievals step before incubation with the antibody?

    The application that is listed on ...

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    My apologies for the late reply, I was away last week. I have received the following information from the source of ab15681: 1. Blocking endogenous biotin is important with the detection system used (biotinylated secondary). This may be improved. ...

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    I'm very sorry to hear you are having a problem with ab15681. This antibody has not been tested in IHC on perfusion formaldehyde fixed sections, we recommend using fresh frozen sections followed by postfixation in glutaraldehyde (0.05%), paraformaldehy...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"