Anti-MHC class I antibody [ERMP42] (ab15680)

Overview

  • Product nameAnti-MHC class I antibody [ERMP42]
    See all MHC class I primary antibodies
  • Description
    Rat monoclonal [ERMP42] to MHC class I
  • SpecificityAb15680 detects murine MHC class I molecules on the surface of cells of the following haplotypes: H-2Fv, H-2Dd,H-2k,q,sp,r,w7,w22. MHC class I molecules of other haplotypes are not recognized by the antibody.
  • Tested applicationsSuitable for: IHC-P, IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse
    Does not react with: Human
  • Immunogen

    murine macrophage precursor cells

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
    Constituents: BSA, PBS
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesPurified IgG from cell culture supernatant.
  • ClonalityMonoclonal
  • Clone numberERMP42
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab15680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration.
IHC-Fr 1/200. Acetone fixation recommended.
Flow Cyt 1/50 - 1/100.

ab18450 - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceMHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.
  • Cellular localizationCell Membrane; Type I membrane protein.
  • Alternative names
    • A 28 antibody
    • A 9 antibody
    • Antigen presenting molecule antibody
    • Aw 24 antibody
    • Aw 68 antibody
    • CLASS I HISTOCOMPATIBILITY ANTIGEN antibody
    • H2 K1 antibody
    • H2K antibody
    • HLA A antibody
    • HLA class I histocompatibility antigen A 1 alpha chain antibody
    • HLA class I histocompatibility antigen A 11 alpha chain antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A 24 alpha chain antibody
    • HLA class I histocompatibility antigen A 3 alpha chain antibody
    • HLA class I histocompatibility antigen A 30 alpha chain antibody
    • HLA class I histocompatibility antigen A 32 alpha chain antibody
    • HLA class I histocompatibility antigen A 68 alpha chain antibody
    • HLAA antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • MHC class I antigen A 1 antibody
    • MHC class I antigen A*11 antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A*24 antibody
    • MHC class I antigen A*3 antibody
    • MHC class I antigen A*30 antibody
    • MHC class I antigen A*32 antibody
    • MHC class I antigen A*68 antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC class I heavy chain H2 K antibody
    see all

References for Anti-MHC class I antibody [ERMP42] (ab15680)

ab15680 has not yet been referenced specifically in any publications.

Product Wall



I contacted the laboratory to clarify the specificity of Anti-MHC class I antibody [ERMP42] (ab15680) for you.

I can confirm that the ab15680 does not react with MHC class I haplotypes in C57BL6 mice. I can recommend reviewing the da...

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ab15680 has indeed been tested in immunohistochemistry on paraffin slides. Although acetone fixation is recommended we have also obtained good results using paraformaldehyde as a fixative. In these cases, we suggest an antigen retrieval step using a ci...

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Thank you for your interesting question. We have some antibodies that recognize one species but not the other, but not all the targets for these antibodies are known to be expressed on these cell lines. What I found were two candidates:

ab15...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Mouse Tissue sections (pancreatic tumor stroma cells)
Specification pancreatic tumor stroma cells
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: citrate
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
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Abcam user community

Verified customer

Submitted Oct 05 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Tissue sections (spleen)
Specification spleen
Fixative Acetone
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted Oct 04 2012

Thank you for your enquiry. This antibody was tested on human tonsil and skin but unfortunately, there isn't any staining on both tissues. Therefore, we have stated on the datasheet that this product does not react with human.

Thank you for your enquiry. I can confirm that indeed ab15680 will cross react with mouse and not human as it is very specific for the mouse protein. Please do not hesitate to contact us again if you require further details about ab15680,

Thank you for your enquiry, we have just received the following information: We have found in our lab that the antibody does not react on routinely fixed paraffin sections (4% formalin). However, from personal communications it seems that the antibo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"